概述| 货号 | PPS003 |
| 别名 | CAMK2; CAMK2B |
全称 | Calcium/Calmodulin-dependent Protein Kinase II | | 应用 | Western Blot(1:1000 dilution)
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| 目标/特异性 | Human, mouse, rat, bovine, chicken, Xenopus ~50 kDa alpha -CaM Kinase II and the ~60 kDa beta -CaM Kinase II phosphorylated at T305 in Western blots. |
| 使用方法 | Western Blot: 1:1000 dilution
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide corresponding to amino acid residues surrounding phospho-T305 of CaM Kinase II |
| 生物活性 | Human, Mouse, Rat, Bovine, Chicken, Xenopus |
| 标记 | Unconjugated |
| 背景 | Calmodulin Kinase II (CaMKII) is a 500 kDa, 8-12 subunit multimer that belongs to the Ser/Thr protein kinase family. It is ubiquitously expressed and interacts with a very diverse group of substrates. In rat, there are four possible subunits/isozymes ( alpha, beta, gamma, δ) that vary from 480-540 amino acids in length. The alpha - and beta -isozymes predominate in the brain. Each subunit contains a catalytic, autoregulatory, and subunit-association domain. The enzyme complex is inactive, due to the association of an internal pseudosubstrate motif with each subunit’s catalytic domain. CaMKII is regulated by calmodulin (CaM), an intracellular receptor for calcium. Following an influx of calcium, two Ca++-CaM complexes interact with inactive CaMKII at the autoregulatory site of two adjacent CaMKII subunits. This dissociates the catalytic site from the pseudosubstrate motif, allowing for the auto(cross)-phosphorylation of T286 on one alpha -subunit (T287 on a beta -subunit) by the catalytic site on an adjacent subunit. The T286 phosphorylation event blocks a reassociation of the catalytic domain with the internal pseudosubstrate motif, resulting in prolonged activation. Once activated, an autoinhibitory program ensues. The dissociation of Ca++-CaM from CaMKII exposes a threonine at position 305. CaMKII autophosphorylation of threonine at this site downregulates existing CaMKII activity. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Griffith, L.C. (2004) J. Neurosci. 24:8391.
- Hudmon, A. and H. Schulman (2002) Annu. Rev. Biochem. 71:473.
- Lin, C.R. et al. (1987) Proc. Natl. Acad. Sci. USA84:5962.
- Thiel, G. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6337.
- Elgersma, Y. et al. (2002) Neuron 36:493.
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参考图片Detection of Phospho-CaM Kinase II (T286)by Western Blot. Western blot of 10 μg of rat brain tissue lysate showing specific immunolabeling of alpha - and beta -CaM Kinase II subunits phosphorylated at T305. The labeling by the antibody to CaM Kinase II (T305) is specifically blocked by the T305 phosphopeptide used as antigen. The corresponding non-phosphopeptide did not block the immunolabeling (not shown). |
