| 货号 | AB4315 |
| 别名 | delta (Drosophila)-like 3; Delta3; delta-like 3 (Drosophila); delta-like protein 3; Drosophila Delta homolog 3; Pudgy; SCDO1; SCDO1delta3 | 全称 | Delta-like 3 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human DLL3 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) DLL1 is observed, and less than 8% cross-reactivity with rhDLL4 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10683 (Human); 13389 (Mouse); 114125 (Rat) |
| 纯化方式 | Protein A or G purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human DLL3 Ala27-Pro488 Accession # Q9NYJ7 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | DLL3 (Delta-like protein 3; also Delta3) is a 65‑67 kDa member of the Delta family of proteins. It is expressed in/on select cell types in the embryo, including somitic mesoderm, cochlear hair cells and noncycling cells of the neural tube that are undergoing differentiation. DLL3 is principally found in the Golgi apparatus and endosomes where it promotes Notch family receptor degradation. Mature human DLL3 is a 592 amino acid (aa) type I transmembrane protein. It possesses a 466 aa extracellular domain (aa 27-492) that contains a Notch-binding DSL domain (aa 176-215) followed by six EGF-like repeats (aa 216-465). There is one isoform variant that contains an Ala substitution for aa 588-618. Over aa 27-488, human DLL3 shares 84% aa identity with mouse DLL3. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human DLL3 by Western Blot. Western blot shows lysates of Hs 294T human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DLL3 Polyclonal Antibody (Catalog # AB4315) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DLL3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of DLL3 in U‑118‑MG Human Cell Line by Flow Cytometry. U‑118‑MG human glioblastoma/astrocytoma cell line was stained with Goat Anti-Human DLL3 Polyclonal Antibody (Catalog # AB4315, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). |
