| 货号 | AB-23-NA |
| 别名 | PDGFA | 全称 | Platelet-derived Growth Factor |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects PDGF in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody will recognize recombinant human (rh) PDGF‑AB, rhPDGF‑AA, rhPDGF‑BB, and natural porcine PDGF‑BB. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize PDGF-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line [Raines, E.W. et al. (1985) Methods Enzymol. 109:749]. The Neutralization Dose (ND50) is typically 3-5 µg/mL in the presence of 10 ng/mL Human PDGF. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5154 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. In vitro differences between venous and arterial-derived smooth muscle cells: potential modulatory role of decorin. | |
| 纯化方式 | Protein A or G purified |
| 免疫原 | Human platelet-derived PDGF |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 1 mg/mL in sterile PBS. |
| 背景 | PDGF was originally discovered as a major mitogenic factor in serum but not in plasma. PDGF is stored in platelet alpha granules and released upon platelet activation. Besides megakaryocytes, other cell types, including endothelial cells, monocyte/macrophages, vascular smooth muscle cells, fibroblasts, cytotrophoblasts and a variety of transformed or neoplastic cells, have been shown to produce PDGF. PDGFs are disulfide-linked dimers. The subunits of the PDGF dimers are homologous polypeptides designated PDGF-A and PDGF-B chains. Natural PDGFs can exist either as homodimers (PDGF-AA, PDGF-BB) or heterodimers (PDGF-AB). Although all three isoforms of PDGF exist in human platelets, R&D Systems hPDGF consists predominantly of hPDGF-AB heterodimers. Two distinct PDGF receptors, the alpha -receptor and the beta -receptor, have been identified. The two receptors are structurally related, with an extracellular portion containing five immunoglobulin-like domains, a single transmembrane region, and an intracellular portion with a protein-tyrosine kinase domain. The alpha -receptor binds both the A and B chains with high affinity whereas the beta -receptor binds only the B-chain with high affinity. Receptor dimerization is induced upon ligand binding. In addition to being a potent mitogen for cells of mesenchymal origin, PDGF has also been shown to be a potent chemoattractant for mesenchymal cells, mononuclear cells and neutrophils and has been reported to be important in the modification of cellular matrix constituents. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
Cell Proliferation Induced by PDGF and Neutralization by PDGF Antibody. Human PDGF (Catalog # 120-HD) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Human PDGF (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-PDGF Polyclonal Antibody (Catalog # AB-23-NA). The ND50 is typically 3-5 µg/mL. |
