| 货号 | APM1676 |
| 别名 | 2 amino 3(4 hydroxyphenyl) propanoic acid; 4 hydroxyphenylalanine |
| 应用 | Western Blot,ELISA |
| 目标/特异性 | Detects proteins containing phosphorylated Tyrosine residues. ELISA and 2D Western blot analyses using pervanadate-treated cell lysates indicate that it binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. This antibody does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues. |
| 使用方法 | Western Blot: 1:1000 dilution ELISA |
| 来源 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| 标记 | Alkaline Phosphatase |
| 溶解方法 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 背景 | Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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ELISA | |
Detection of Phospho-Tyrosine by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1:1000 dilution of Phospho-Tyrosine AP-conjugated Monoclonal Antibody (Catalog # APM1676), followed by [Secondary Antibody Proper Name & Link]. Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX. |
