Phospho-Tyrosine Agarose MAb (Clone 179003) (250 UL)
货号：
AGM1676 基本售价：
4243.4 元 规格：
-
产品信息
概述| 货号 | AGM1676 |
| 别名 | 2 amino 3(4 hydroxyphenyl) propanoic acid; 4 hydroxyphenylalanine |
| 应用 | Immunoprecipitation |
| 目标/特异性 | Detects endogenous proteins containing phosphorylated Tyrosine residues. ELISA and 2D Western blot analyses using ligand- and pervanadate-treated cell lysates indicate that clone 179003 binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. Does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues. |
| 使用方法 | Immunoprecipitation: 10 µL/500 µg cell lysate
|
| 来源 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| 标记 | Agarose |
| 溶解方法 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 背景 | Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - GDNF Family
- New Tools: Phospho-Tyrosine Antibodies
|
参考图片Immunoprecipitation of Human Phospho-tyrosine. A431 human epithelial carcinoma cell line was untreated (-) or treated (+) with 200 mM pervanadate (PV) for ???time. Phospho-tyrosine was immunoprecipitated from 500 μg of cell lysate following incubation with 10 µg Mouse Anti-Phospho-Tyrosine Agarose-conjugated Monoclonal Antibody (Catalog # AGM1676) for 16 hours at 2 - 8 ºC. Immunoprecipitated phospho-tyrosine was detected by Western blot using ??? µg Phospho-Tyrosine Biotinylated Monoclonal Antibody (left panel, Catalog # BAM1676) or Human EGF R/ErbB1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (right panel, Catalog # BAF231). View our recommended buffer recipes for immunoprecipitation. |
