| 货号 | PA-ST6 |
| 别名 | D12S1644; EC 2.4.1.227; EC 2.7.7.6; IL-4 Stat; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin4-induced; STAT6B; STAT6C; transcription factor IL-4 STAT | 全称 | Signal Transducer and Activator of Transcription 6 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(2000x µg/mL) Immunoprecipitation(3-6 µg/106cells) |
| 目标/特异性 | Detects human and murine STAT 6. This antiserum does not crossreact with any of the other STAT proteins. |
| 使用方法 | Western Blot: 2000x µg/mL Immunoprecipitation: 3-6 µg/106cells |
| 来源 | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6778 (Human); 20852 (Mouse); 362896 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Type 2 diabetes impairs insulin receptor substrate-2-mediated phosphatidylinositol 3-kinase activity in primary macrophages to induce a state of cytokine resistance to IL-4 in association with overexpression of suppressor of cytokine signaling-3. | |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 背景 | Signal Transducer and Activator of Transcription 6 (STAT6) is a member of the Jak/STAT signal transduction pathway and mediates cytokine signaling by IL-4. IL-4 activation leads to tyrosine phosphorylation and accumulation in the nucleus of STAT6 homo and heterodimers. STAT6 acts as a signal transducer in the cytoplasm and, upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN4GAA. Knockout studies in mice suggest that STAT6 functions in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. STAT6 transcriptional activation occurs by binding p100 and p160 class (NcoA1, Src-1) co-activators. Gene expression studies indicate that STAT6 mediates cell proliferation and anti-apoptotic effects of IL-4. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human STAT6 by Western Blot. Western blot shows lysates of TF‑1 human erythroleukemic cell line and human peripheral blood lymphocyte. PVDF membrane was probed with 1:2000 dilution of Human/Mouse STAT6 Polyclonal Antibody (Catalog # PA-ST6) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT6 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9. |
