Human/Mouse/Rat p53 HRP Affinity Purified Polyclonal Ab (100 UL)【科瑞奥博】

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Human/Mouse/Rat p53 HRP Affinity Purified Polyclonal Ab (100 UL)

货号： HAF1355 基本售价： 4164.1 元规格： -

产品信息

概述
货号	HAF1355
别名	Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53
反应种属	Human/Mouse/Rat
应用	Western Blot(1:5000 dilution)
目标/特异性	Detects human, mouse, and rat p53 in Western blots.
使用方法	Western Blot: 1:5000 dilution
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7157 (Human); 22059 (Mouse); 24842 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The MBT repeats of L3MBTL1 link SET8-mediated p53 methylation at lysine 382 to target gene repression. Authors: West LE, Roy S, Lachmi-Weiner K J. Biol. Chem., 2010;285(48):37725-32. Species: Human Sample Type: Cell Lysates Application: WB KRAB-type zinc-finger protein Apak specifically regulates p53-dependent apoptosis. Authors: Tian C, Xing G, Xie P, Lu K, Nie J, Wang J, Li L, Gao M, Zhang L, He F Nat. Cell Biol., 2009;11(5):580-91. Species: Human Sample Type: Cell Lysates Application: WB Arginine methylation of hnRNP K enhances p53 transcriptional activity. Authors: Chen Y, Zhou X, Liu N, Wang C, Zhang L, Mo W, Hu G FEBS Lett., 2008;582(12):1761-5. Species: Human Sample Type: Cell Lysates Application: WB Histone methyltransferase protein SETD2 interacts with p53 and selectively regulates its downstream genes. Authors: Xie P, Tian C, An L, Nie J, Lu K, Xing G, Zhang L, He F Cell. Signal., 2008;20(9):1671-8. Species: Human Sample Type: Cell Lysates Application: WB Role for 53BP1 Tudor domain recognition of p53 dimethylated at lysine 382 in DNA damage signaling. Authors: Kachirskaia I, Shi X, Yamaguchi H, Tanoue K, Wen H, Wang EW, Appella E, Gozani O J. Biol. Chem., 2008;283(50):34660-6. Species: N/A Sample Type: recombinant p53 Application: WB SVH-B interacts directly with p53 and suppresses the transcriptional activity of p53. Authors: Zhou X, Yang G, Huang R, Chen X, Hu G FEBS Lett., 2007;581(25):4943-8. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human p53 Asp7-Asp393 Accession # P04637
生物活性	Human, Mouse, Rat
标记	HRP
背景	The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.
运输条件	Blue Ice
存放说明	4℃
参考文献	Apoptosis Transcription Factors and Regulators Cancer Biomarkers Cancer Stem Cell Transcription Factors Cell Cycle Checkpoint DNA Damage and Repair Epithelial Stem Cell Transcription Factors Hematopoietic Stem Cell Transcription Factors and Regulators ICC / IHC Images: p53 Immunoglobulin-Like Domain Transcription Factors Mesenchymal Stem Cell Transcription Factors Mini-review: DNA Damage Response Mini-review: Genomic Instability Syndromes Neural Stem Cell Transcription Factors p53 Pathway p53: More than a Transcription Factor? Parkinsons Disease Related Molecules Pluripotent Stem Cell Transcription Factors TNF Superfamily Regulators Transcription Factors and Regulators Transcription Factors in the Akt Pathway

参考图片

Detection of Human p53 by Western Blot. Western blot shows lysates of CEM human T‑lymphoblastoid cell line and MCF‑7 human breast cancer cell line untreated
(-) or treated (+) with 10 Gy ionizing radiation (IR) for 1 hour. PVDF membrane was probed with 1:5000 dilution of Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # HAF1355). A specific band was detected for p53 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.