Human Phospho-STAT4 (Y693) Affinity Purified PAb (100 UG)
货号：
AF4319 基本售价：
4094.7 元 规格：
-
产品信息
概述| 货号 | AF4319 |
| 别名 | signal transducer and activator of transcription 4; SLEB11 |
全称 | Signal Transducer and Activator of Transcription 4 | | 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL)
|
| 目标/特异性 | Detects human STAT4 when phosphorylated at Y693. |
| 使用方法 | Western Blot: 1 µg/mL
|
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 6775 (Human); 20849 (Mouse) |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Human memory, but not naive, CD4+ T cells expressing transcription factor T-bet might drive rapid cytokine production.
Authors: Yu S, Zhang Y, Yang B, Wu C
J Biol Chem, 2014;289(51):35561-9.
Species: Human
Sample Type: Cell Lysates
Application: WB
Human invariant natural killer T cells acquire transient innate responsiveness via histone H4 acetylation induced by weak TCR stimulation.
Authors: Wang X, Bishop KA, Hegde S, Rodenkirch LA, Pike JW, Gumperz JE
J. Exp. Med., 2012;209(5):987-1000.
Species: Human
Sample Type: Whole Cells
Application: Flow |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide containing human Phospho-STAT4 Y693 site |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Signal transduction and activator of transcription 4 (STAT4) is a member of the Jak/STAT signal transduction pathway. STAT4 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, homo or heterodimerizes with other STAT proteins, which then translocate to the nucleus and activate transcription of specific genes. STAT4 is expressed in subset of tissues including spleen, heart, brain, peripheral blood cells and testis. STAT4 is involved in mediating IL-12 response in lymphocytes and regulating T helper cell differentiation. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Apoptosis Transcription Factors and Regulators
- Hematopoietic Stem Cell Transcription Factors and Regulators
- IL-12 Family
- Immunoglobulin-Like Domain Transcription Factors
- Interactive Pathway: Th1 Differentiation
- Jak and STAT Proteins
- Mini-review: STAT Proteins
- STAT
- Th1 Cells
|
参考图片Detection of Human Phospho-STAT4 (Y693) by Western Blot. Western blot shows lysates of NC‑37 human Burkitts lymphoma B lymphoblast cell line untreated (-) or treated (+) with pervanadate for 5 minutes. PVDF membrane was probed with 1 µg/mL Human Phospho-STAT4 (Y693) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4319) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-STAT4 (Y693) was detected at approximately 95 kDa (as indicated). For additional reference, duplicate lysates were probed with 1.0 µg/mL Human/Mouse STAT4 Polyclonal Antibody ( lower panel, Catalog # PA-ST4). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
