| 货号 | AF5126 |
| 别名 | FGFR signalling adaptor; FGFR-signaling adaptor SNT; fibroblast growth factor receptor substrate 2; FRS2A; FRS2alpha; SNT; SNT1; SNT-1FGFR substrate 2; Suc1-associated neurotrophic factor target 1 | 全称 | Fibroblast Growth Factor Receptor Substrate 2 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat FRS2 isoforms when phosphorylated at Y436. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10818 (Human); 327826 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Activation of the FGFR-STAT3 pathway in breast cancer cells induces a hyaluronan-rich microenvironment that licenses tumor formation. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide containing human FRS2 Y436 site |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | FRS2 (FGF R substrate 2; also known as SNT and FRS2-alpha ) is a 70‑90 kDa member of the FRS family of lipid-anchored docking proteins. It is an intermediary between FGF and RTK receptors and their Ras/MAPK signaling cascades. FRS2 contains a membrane-anchoring myristoylation signal (aa 1‑6), a PTB domain that interacts with FGF and NGF receptors (aa 13‑115), and a C-terminal tyrosine-rich region that serves as a docking site for GRB-2 and SHP-2 (aa 196‑471). Phosphorylation of Y436 by activated RTKs is required for efficient SHP-2 recruitment. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human and Rat Phospho-FRS2 (Y436) by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes and PC‑12 rat adrenal pheochromocytoma cell line untreated or treated with 100 ng/mL Recombinant Rat beta -NGF (Catalog #556-NG) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-FRS2 (Y436) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5126), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Bands were detected for Phospho-FRS2 (Y436) at approximately 70 - 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Phospho-FRS2 (Y436) in A431 Human Cell Line. FRS2 phosphorylated at Y436 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human/Mouse/Rat Phospho-FRS2 (Y436) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5126) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
