| 货号 | AF3989 |
| 别名 | EC 3.1.3.16; PP2A-alpha; PP2Ac; PP2CA; PP2Calpha; PPP2CA; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2A catalytic subunit, alpha isoform; Replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat PP2A when phosphorylated at Y307. The antigen is identical to the PP4 Y305 phosphorylation site. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5515 (Human); 51792 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Sulfiredoxin-Peroxiredoxin IV axis promotes human lung cancer progression through modulation of specific phosphokinase signaling. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide containing human PP2A Y307 site |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Protein Phosphatase 2A (PP2A) dephosphorylates serine and threonine residues in proteins. This ubiquitously expressed enzyme plays a critical role in modulating cell survival, growth factor responses, and neurotransmission. Phosphorylation near the C-terminus at Y307 of the catalytic subunit decreases the phosphatase activity of PP2A and reduces its ability to bind to proteins such as CD28 and the glutamate receptor GluR5. Phosphorylation of PP2A at Y307 is highest during M-phase and lowest during S-phase of the cell cycle. Transient PP2A phosphorylation at this site is also observed in growth factor-stimulated cells. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human Phospho-PP2A (Y307), Catalytic Subunit by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with hydroxyurea. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3989), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PP2A (Y307), Catalytic Subunit at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
