| 货号 | AF888 |
| 别名 | CDC28A; CDC2MGC111195; CDK1; cell cycle controller CDC2; Cell division control protein 2 homolog; Cell division protein kinase 1; cyclin-dependent kinase 1; DKFZp686L20222; EC 2.7.11.22; EC 2.7.11.23; G1 to S and G2 to M; p34 protein kinase; P34CDC2 | 全称 | Cell Division Cycle 2/Cyclin-dependent Kinase 1 |
| 应用 | Western Blot(0.2 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat CDC2 when phosphorylated at Y15. |
| 使用方法 | Western Blot: 0.2 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 983 (Human); 12534 (Mouse); 54237 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Unique functions of CHK1 and WEE1 underlie synergistic anti-tumor activity upon pharmacologic inhibition. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide containing CDC2 Y15 site |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CDC2 (Cell Division Cycle 2), also known as CDK1 (Cyclin Dependent Kinase 1), is a member of the CDK family of serine/threonine kinases. The CDKs are important regulators of cell cycle progression and their activities are largely controlled by association with Cyclins, and activating and inhibitory phosphorylations. Entry into mitosis is initiated by CDC2. Full activation of CDC2 requires phosphorylation at T161 and association with CyclinB. In contrast, phosphorylation of CDC2 at Y15 and T14 during the G2-phase of the cell cycle inhibits activity, and dephosphorylation of Y15 and T14 by CDC25 phosphatase during late G2 restores activity. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Mouse and Human Phospho-CDC2 (Y15) by Western Blot. Western blot shows lysates of L‑929 mouse fibroblast cell line and Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 0.2 μg/mL nocodazole or 12 μM aphidicolin or 1 mM hydroxurea for 18 hours. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-CDC2 (Y15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF888), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-CDC2 (Y15) at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
