概述| 货号 | AF5817 |
| 别名 | c-erbB3; EC 2.7.10; EC 2.7.10.1; ErbB-3; erbB3-S; HER3; HER3c-erbB-3; LCCS2; lethal congenital contracture syndrome 2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) |
全称 | Receptor Tyrosine Protein Kinase ErbB3 | | 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL)
|
| 目标/特异性 | Detects human ErbB3 when phosphorylated at Y1262 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL
|
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 2065 (Human); 13867 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide containing human ErbB3 Y1262 site |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ErbB3 (also known as HER3) is a type I transmembrane growth factor receptor and member of the human epidermal growth factor receptor (HER) family. ErbB3 is unique among HER family members in that it contains a defective kinase domain, and is therefore active only as a partner in a heterodimer complex with other ErbBs. Despite ErbB3s lack of intrinsic kinase activity, these heterodimers can be quite potent: the ErbB2/ErbB3 complex is considered the most active ErbB signaling dimer for the activation of downstream pathways. In addition, monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG), while ErbB2/ErbB3 heterodimers form a high affinity receptor complex. Phosphorylation of ErbB3 at Y1262 following ligand binding generates a binding site for the Grb2 adaptor protein. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Cancer Biomarkers
- EGF Family
- ICC / IHC Images: ErbB3/Her3
- Receptor Tyrosine Kinases (RTKs)
- Receptor Tyrosine Kinases (RTKs) in the Akt Pathway
- The ErbB Family - Talk and Crosstalk
|
参考图片Detection of Human Phospho-ErbB3/Her3 (Y1262) by Western Blot. Western blot shows lysates of MDA‑MB‑453 human breast cancer cell line untreated (-) or treated (+) with 300 ng/mL Recombinant Human NRG1-beta /HRG1-beta (Catalog # 396-HB) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Human Phospho-ErbB3/Her3 (Y1262) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5817), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-ErbB3/Her3 (Y1262) at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
