Human Phospho-VEGF R1/Flt-1 (Y1213) Affinity Purified PAb (100 UG)【科瑞奥博】

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Human Phospho-VEGF R1/Flt-1 (Y1213) Affinity Purified PAb (100 UG)

货号： AF4170 基本售价： 4094.7 元规格： -

产品信息

概述
货号	AF4170
别名	EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGFR1; VEGFR-1
全称	Vascular Endothelial Growth Factor Receptor 1
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human VEGF R1/Flt-1 when phosphorylated at Y1213.
使用方法	Western Blot: 1 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2321 (Human); 14254 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Deletion of placental growth factor prevents diabetic retinopathy and is associated with Akt activation and HIF1alpha-VEGF pathway inhibition. Authors: Huang H, He J, Johnson D, Wei Y, Liu Y, Wang S, Lutty G, Duh E, Semba R Diabetes, 2015;64(1):200-12. Species: Mouse Sample Type: tissue Application: IF Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophages. Authors: Meda C, Molla F, De Pizzol M J. Immunol., 2012;188(8):4081-92. Species: Human Sample Type: Cell Lysates Application: WB Bevacizumab attenuates major signaling cascades and eIF4E translation initiation factor in multiple myeloma cells. Authors: Attar-Schneider O, Drucker L, Zismanov V Lab. Invest., 2012;92(2):178-90. Species: Human Sample Type: Cell Lysates Application: WB Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1. Authors: Ponticelli S, Marasco D, Tarallo V, Albuquerque RJ, Mitola S, Takeda A, Stassen JM, Presta M, Ambati J, Ruvo M, De Falco S J. Biol. Chem., 2008;283(49):34250-9. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Phosphopeptide containing human VEGF R1/Flt-1 Y1213 site
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	VEGF R1, also known as Flt-1, is a receptor tyrosine kinase that contains seven Ig-like repeats in its extracellular domain, a single transmembrane region, and a kinase domain in the intracellular region. Ligand binding induces autophosphorylation on multiple intracellular tyrosine residues. Y1213 is a major binding site for PI3K, Nck, and SHP-2.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Airway Inflammation and Remodeling Cancer Biomarkers Endothelial Cell Differentiation Markers Endothelial Cell Markers Endothelial Progenitor and Endothelial Cell Markers HIF Transcription Factors ICC / IHC Images: VEGF R1/Flt-1 Mini-review: Vascular Endothelial Growth Factor (VEGF) Myeloid-derived Suppressor Cells (MDSC) Platelets Preeclampsia Proliferation Bioassay HUVECs Receptor Tyrosine Kinases (RTKs) Receptor Tyrosine Kinases (RTKs) in the Akt Pathway Receptors in the Jak/STAT Pathway Technical Notes: The Quantikine ELISA in Establishing a Role for Endoglin in Preeclampsia VEGF Family

参考图片

Detection of Human Phospho-VEGF R1/Flt‑1 (Y1213) by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cell untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-VEGF R1/Flt‑1 (Y1213) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4170), followed by HRP-conjugated Anti‑Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-VEGF R1/Flt‑1 (Y1213) at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.