概述| 货号 | AF7605 |
| 别名 | EIF2AK4; eukaryotic translation initiation factor 2 alpha kinase 4; eukaryotic translation initiation factor 2-alpha kinase 4; GCN2GCN2 eIF2alpha kinase; GCN2-like protein; KIAA1338EC 2.7.11.1 |
全称 | General Control Nonrepressed 2-like Protein | | 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL)
|
| 目标/特异性 | Detects human GCN2 when phosphorylated at T899 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL
|
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 440275 (Human); 27103 (Mouse); 114859 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide containing human GCN2 T899 site |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | GCN2 (General Control Non-derepressible 2-like protein; alsoeIF2a kinase 4) is a 190-200kDa member of the GCN2 subfamily,Ser/Thr kinase family, protein kinase superfamily of proteins It is widely expressed, being found inskeletal muscle cells, fibroblasts, hepatocytes and neurons. GCN2 is a key regulator of metabolism undernutrient deprivation. In particular,when essential amino acids (aa) are limiting, tRNAs exist that are unbound toamino acids. These “uncharged” tRNAswill bind to GCN2, activating the molecule. Through GCN2-mediated phosphorylation of eIF2a, promotes GCN4 synthesis,with subsequent activation of amino acid synthesizing enzymes, and adownregulation of lipid storage. Human GCN2is 1649 aa in length.It possesses one RWD domain (aa 25-137), two protein kinase domains (aa296-539 and 590-1001) and an extended His-RS-like region (aa 1021-1492). There are at least three utilized Ser/Thrphosphorylation sites. Two isoformvariants exist. One shows a deletion ofaa 774-801, while another contains a nine aa substitution for aa 608-1649. Over aa 22-139, human GCN2 shares 91% aasequence identity with mouse GCN2. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Translation Regulation by the Akt Pathway
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参考图片Detection of Human Phospho-GCN2 (T899) by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 20 mJ/cm 2 ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-GCN2 (T899) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7605) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho‑GCN2 (T899) at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
