Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Aff Pur PAb (100 UG)【科瑞奥博】

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Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Aff Pur PAb (100 UG)

货号： AF1018 基本售价： 4094.7 元规格： -

产品信息

概述
货号	AF1018
别名	ERK1/ERK2
全称	Extracellular Signal-regulated Kinase 1/2
应用	Western Blot(0.1 µg/mL) Simple Western(5 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human, mouse and rat Phospho-ERK1/ERK2 when dually phosphorylated at T202/Y204 and T185/Y187, respectively.
使用方法	Western Blot: 0.1 µg/mL Simple Western: 5 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Sirtuin 3 (SIRT3) Regulates ?-Smooth Muscle Actin (?-SMA) Production through the Succinate Dehydrogenase-G Protein-coupled Receptor 91 (GPR91) Pathway in Hepatic Stellate Cells J Biol Chem, 2016;291(19):10277-92. Species: Human Sample Type: Cell Lysates Application: WB Ability of the Met kinase inhibitor crizotinib and new generation EGFR inhibitors to overcome resistance to EGFR inhibitors. Authors: Nanjo, Shigeki, Yamada, Tadaaki, Nishihara, Hiroshi, Takeuchi, Shinji, Sano, Takako, Nakagawa, Takayuki, Ishikawa, Daisuke, Zhao, Lu, Ebi, Hiromich, Yasumoto, Kazuo, Matsumoto, Kunio, Yano, Seiji PLoS ONE, 2013;8(12):e84700. Species: Human Sample Type: Cell Lysate Application: WB Influence of gefitinib and erlotinib on apoptosis and c-MYC expression in H23 lung cancer cells. Authors: Suenaga M, Yamamoto M, Tabata S, Itakura S, Miyata M, Hamasaki S, Furukawa T Anticancer Res, 2013;33(4):1547-54. Species: Human Sample Type: Cell Lysates Application: WB Perlecan Domain V induces VEGF secretion in brain endothelial cells through integrin alpha5beta1 and ERK-dependent signaling pathways. Authors: Clarke D, Al Ahmad A, Lee B, Parham C, Auckland L, Fertala A, Kahle M, Shaw C, Roberts J, Bix G PLoS ONE, 2012;7(9):e45257. Species: Human Sample Type: Cell Lysates Application: WB The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer. Authors: Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S Clin. Cancer Res., 2011;17(11):3619-30. Species: Human Sample Type: Cell Lysates Application: WB Transient PI3K Inhibition Induces Apoptosis and Overcomes HGF-Mediated Resistance to EGFR-TKIs in EGFR Mutant Lung Cancer. Authors: Donev IS, Wang W, Yamada T, Li Q, Takeuchi S, Matsumoto K, Yamori T, Nishioka Y, Sone S, Yano S Clin. Cancer Res., 2011;17(8):2260-9. Species: Human Sample Type: Cell Lysates Application: WB Deoxygedunin, a natural product with potent neurotrophic activity in mice. Authors: Jang SW, Liu X, Chan CB PLoS ONE, 2010;5(7):e11528. Species: Mouse Sample Type: Cell Lysates Application: WB CB(2) cannabinoid receptor activation is cardioprotective in a mouse model of ischemia/reperfusion. Authors: Montecucco F, Lenglet S, Braunersreuther V, Burger F, Pelli G, Bertolotto M, Mach F, Steffens S J. Mol. Cell. Cardiol., 2009;46(5):612-20. Species: Mouse Sample Type: Tissue Homogenates Application: WB Long-term high glucose concentration influences Akt, ERK1/2, and PTP1B protein expression in human aortic smooth muscle cells. Authors: Popov D, Nemecz M, Dumitrescu M, Georgescu A, Bohmer FD Biochem. Biophys. Res. Commun., 2009;388(1):51-5. Species: Human Sample Type: Cell Lysates Application: WB E-selectin regulates gene expression in metastatic colorectal carcinoma cells and enhances HMGB1 release. Authors: Aychek T, Miller K, Sagi-Assif O, Levy-Nissenbaum O, Israeli-Amit M, Pasmanik-Chor M, Jacob-Hirsch J, Amariglio N, Rechavi G, Witz IP Int. J. Cancer, 2008;123(8):1741-50. Species: Human Sample Type: Cell Lysates Application: WB The RAS/Raf1/MEK/ERK signaling pathway facilitates VSV-mediated oncolysis: implication for the defective interferon response in cancer cells. Authors: Noser JA, Mael AA, Sakuma R, Ohmine S, Marcato P, Lee PW, Ikeda Y Mol. Ther., 2007;15(8):1531-6. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Phosphopeptide containing ERK1 T202/Y204 sites
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	ERK1 is a protein Serine/Threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain, and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Roskoski Jr., R. (2012) Pharmacol Res. 66:105.

参考图片

Detection of Human Phospho-ERK1 (T202/Y204) and ERK2 (T185/Y187) by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for for the indicated times. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho‑ERK1 (T202/Y204)/ERK2 (T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho‑ERK1 (T202/Y204) and ERK2 (T185/Y187) at approximately 42 and 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Phospho-ERK1/ERK2 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line were untreated (yellow line open histogram) or treated with 200 ng/mL PMA for 15 minutes (filled histogram) and stained with Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018) or control antibody (Catalog # AB‑105‑C, blue line open histogram), followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with PFA and permeabilized with ice-cold methanol.

ERK1/ERK2 in Rat Brain.

ERK1/ERK2 was detected in perfusion fixed frozen sections of rat brain (cortex) using 15 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018) overnight at 4 °C. Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with PMA, loaded at 0.2 mg/mL. A specific band was detected for ERK1 (T202/Y204)/ERK2 (T185/Y187) at approximately 44 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.