Human Phospho-PGC1 alpha (S571) Affinity Purified PAb (100 UG)【科瑞奥博】

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Human Phospho-PGC1 alpha (S571) Affinity Purified PAb (100 UG)

货号： AF6650 基本售价： 4094.7 元规格： -

产品信息

概述
货号	AF6650
别名	LEM6; Ligand effect modulator 6; ligand effect modulator-6; peroxisome proliferative activated receptor, gamma, coactivator 1, alpha; peroxisome proliferator-activated receptor gamma coactivator 1-alpha; peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; PGC1A; PGC-1-alpha; PGC1APGC-1(alpha); PGC1peroxisome proliferative activated receptor, gamma, coactivator 1; PPAR gamma coactivator variant form; PPAR gamma coactivator-1; PPAR-gamma coactivator 1-alpha; PPARGC1A; PPARGC-1-alpha; PPARGC1PGC-1v
全称	Peroxisome Proliferator-activated Receptor gamma, Coactivator 1 alpha
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human PGC1 alpha when phosphorylated at S571 in Western blots.
使用方法	Western Blot: 1 µg/mL
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10891 (Human); 19017 (Mouse); 83516 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Metabolic Reprogramming Is Required for Myofibroblast Contractility and Differentiation. Authors: Bernard K, Logsdon N, Ravi S, Xie N, Persons B, Rangarajan S, Zmijewski J, Mitra K, Liu G, Darley-Usmar V, Thannickal V J Biol Chem, 2015;290(42):25427-38. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Phosphopeptide containing the human PGC1 alpha S571 site.
生物活性	Human
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	PGC-1 alpha (PPAR-gamma coactivator 1; also LEM6) is a 97-120 kDa member of the PGC-1 family of proteins. It is expressed in select cell types, including brown adipocytes, skeletal muscle and hepatocytes. PGC-1 alpha participates in both RNA processing and transcriptional coactivation in conjunction with multiple nuclear hormone receptors such as PPAR gamma, RAR and TR. Human PCG-1 alpha is 798 amino acids (aa) in length. It contains an LxxLL nuclear receptor binding motif (aa 144-148), one PPAR-gamma interaction domain (aa 293-339), two NLSs and an RNA binding/processing region (aa 566-710). PGC-1 alpha activity is regulated by phosphorylation. AMPK is known to phosphorylate Thr178 and Ser539, promoting cotranscriptional activity. Conversely, Akt-mediated phosphorylation at Ser571 is reported to downregulate PGC-1 alpha activity. This latter effect is achieved by an initial Ser571 phosphorylation, followed by GCN5 binding and subsequent PCG-1 alpha acetylation that promotes PGC-1 alpha dissociation from target gene promoters.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Adipogenesis Markers Adiponectin Signaling: The Calcium Connection Glucose Homeostasis Nuclear Hormone Receptors and Regulators Other Lipid/Cholesterol Metabolism Molecules Stem Cell Transcription Factor Regulators Transcriptional Co-Activators

参考图片

Detection of Human Phospho-PGC1 alpha (S571) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 10 nm Recombinant Human IGF‑I (Catalog # 291-G1) for 1 hour. PVDF Membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-PGC1 alpha (S571) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6650) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PGC1 alpha (S571) at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.