Phospho-Akt (S473) Pan Specific Affinity Purified Pab (100 UG)【科瑞奥博】

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Phospho-Akt (S473) Pan Specific Affinity Purified Pab (100 UG)

货号： AF887 基本售价： 4094.7 元规格： -

产品信息

概述
货号	AF887
别名	PKB; RAC
全称	v-Akt Murine Thymoma Viral Oncogene Homolog
应用	Western Blot(0.5 µg/mL) Simple Western(5 µg/mL) Immunohistochemistry(5-15 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human, mouse and rat Akt1, Akt2 and Akt3, when phosphorylated at S473, S474 and S472, respectively.
使用方法	Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL Immunohistochemistry: 5-15 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Dual inhibition of CDK4/Rb and PI3K/AKT/mTOR pathways by ON123300 induces synthetic lethality in mantle cell lymphomas. Authors: Divakar S, Ramana Reddy M, Cosenza S, Baker S, Perumal D, Antonelli A, Brody J, Akula B, Parekh S, Premkumar Reddy E Leukemia, 2016;30(1):86-93. Species: Human Sample Type: Cell Lysates Application: WB Interferon-gamma suppresses intestinal epithelial aquaporin-1 expression via Janus kinase and STAT3 activation. Authors: Dicay M, Hirota C, Ronaghan N, Peplowski M, Zaheer R, Carati C, MacNaughton W PLoS ONE, 2015;10(3):e0118713. Species: Mouse Sample Type: Cell Lysates Application: WB The role of nuclear beta-catenin accumulation in the Twist2-induced ovarian cancer EMT. Authors: Mao Y, Xu J, Li Z, Zhang N, Yin H, Liu Z PLoS ONE, 2013;8(11):e78200. Species: Human Sample Type: Cell Lysates Application: WB An elaborate regulation of Mammalian target of rapamycin activity is required for somatic cell reprogramming induced by defined transcription factors. Authors: He, Jing, Kang, Lan, Wu, Tong, Zhang, Jiqin, Wang, Haitao, Gao, Haibo, Zhang, Yu, Huang, Bo, Liu, Wenqiang, Kou, Zhaohui, Zhang, Hongbing, Gao, Shaorong Stem Cells Dev, 2012;21(14):2630-41. Species: Mouse Sample Type: Cell Lysates Application: WB Single amino Acid substitutions in the chemotactic sequence of urokinase receptor modulate cell migration and invasion. Authors: Bifulco, Katia, Longanesi-Cattani, Immacola, Franco, Paola, Pavone, Vincenzo, Mugione, Pietro, Di Carluccio, Gioconda, Masucci, Maria Te, Arra, Claudio, Pirozzi, Giuseppe, Stoppelli, Maria Pa, Carriero, Maria Vi PLoS ONE, 2012;7(9):e44806. Species: Human Sample Type: Cell Lysates Application: WB EphB receptors trigger Akt activation and suppress Fas receptor-induced apoptosis in malignant T lymphocytes. Authors: Maddigan A, Truitt L, Arsenault R J. Immunol., 2011;187(11):5983-94. Species: Human Sample Type: Cell Lysates Application: WB Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation. Authors: Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, Kang J, Zhang X, Yan C J. Mol. Cell. Cardiol., 2010;48(6):1225-35. Species: Human Sample Type: Cell Lysates Application: WB Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt. Authors: Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, Li S Apoptosis, 2010;15(4):463-73. Species: Rat Sample Type: Cell Lysates Application: WB The involvement of the fractalkine receptor in the transmigration of neuroblastoma cells through bone-marrow endothelial cells. Authors: Nevo I, Sagi-Assif O, Meshel T, Ben-Baruch A, Johrer K, Greil R, Trejo LE, Kharenko O, Feinmesser M, Yron I, Witz IP Cancer Lett., 2009;273(1):127-39. Species: Human Sample Type: Cell Lysates Application: WB Osteoblast-derived factors induce an expression signature that identifies prostate cancer metastasis and hormonal progression. Authors: Wang G, Haile S, Comuzzi B, Tien AH, Wang J, Yong TM, Jelescu-Bodos AE, Blaszczyk N, Vessella RL, Masri BA, Sadar MD Cancer Res., 2009;69(8):3433-42. Species: Human Sample Type: Cell Lysates Application: WB CREG inhibits migration of human vascular smooth muscle cells by mediating IGF-II endocytosis. Authors: Han Y, Cui J, Tao J, Guo L, Guo P, Sun M, Kang J, Zhang X, Yan C, Li S Exp. Cell Res., 2009;315(19):3301-11. Species: Human Sample Type: Cell Lysates Application: WB Enhancing mammalian target of rapamycin (mTOR)-targeted cancer therapy by preventing mTOR/raptor inhibition-initiated, mTOR/rictor-independent Akt activation. Authors: Wang X, Yue P, Kim YA, Fu H, Khuri FR, Sun SY Cancer Res., 2008;68(18):7409-18. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen and protein A Affinity-purified
免疫原	Phosphopeptide containing human, mouse, and rat Akt1 S473 site
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Akt1, Akt2, and Akt3 make up a family of serine/threonine kinases, often activated downstream of growth factor receptors and PI 3-Kinase. Each Akt family member contains an N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a C-terminal regulatory domain. They have putative roles in cell proliferation and survival, and Akt perturbations are thought to play an important role in tumorigenesis.
运输条件	Blue Ice
存放说明	-20℃
参考文献	A Novel Pathway for TOR-independent Autophagy Regulation by Akt Akt Modulates TGF-beta Activities Apoptosis Intracellular Kinases Cancer Biomarkers Chemokine Receptor Intracellular Signaling Common gamma Chain Receptor Family Diabetic Peripheral Neuropathy GDNF Family Human Phospho-MAPK Array Kit ICC / IHC Images: Akt Interactive Pathway: Akt Signaling Pathway Intracellular Kinases Intracellular Kinases in the Akt Pathway Intracellular Signaling Molecules in Angiogenesis Multiple Myeloma Neurotrophin/Trk Family Notch Pathway PDGF Family Signaling Molecules and Transcriptional Regulators of Tfh Cell Differentiation VEGF Family

参考图片

Detection of Mouse and Human Phospho-Akt (S473) Pan Specific by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog #120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog #291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Akt in Human Breast Cancer Tissue. Akt phosphorylated at S473 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Phospho-Akt (S473) in IGF-1-treated MCF‑7 cells by Flow Cytometry. Serum-starved MCF‑7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho‑Akt (S473) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF887) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Detection of Human Phospho-Akt (S473) by Simple Western^TM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.