| 货号 | AF5955 |
| 别名 | NR3C3; NR3C3PRNuclear receptor subfamily 3 group C member 3; PGR; progesterone receptor | 全称 | Progesterone Receptor |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human Phospho-Progesterone R/NR3C3 (S294) in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5241 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide containing human Progesterone RB S294 site Accession # P06401 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The progesterone Receptor (PR; NR3C3) is a member of the steroid receptor superfamily which binds progesterone. The progesterone receptor is expressed in uterus, ovary, vagina, fallopian tubes and breast. Progesterone receptors play a central role in diverse reproductive events associated with the establishment and maintenance of pregnancy, alveolar development in the breast, and sexual behavior. Two isoforms of the progesterone receptor have been identified, progesterone receptor-A (PR-A) and progesterone receptor-B (PR-B), 94 and 114 kDa in size, respectively. The B progesterone receptor (PR-B), or long form, is 933 amino acids (aa) in length. Progesterone receptor-A (PR-A) utilizes a different start site that shortens the N-terminus by 164 amino acids. The N-terminus in both progesterone receptor isoforms is rich in serine that is phosphorylated in response to hormone binding. In the absence of progesterone, few receptor molecules are phosphorylated at Serine 190 (S190). Progesterone increases this number two-fold, providing evidence for hormone stimulation. It has been shown that the two progesterone receptor isoforms can form homodimers as well as heterodimers. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human Phospho-Progesterone RB (S294) and Phospho-Progesterone RA (S130) by Western Blot. Western blot shows lysates of T47D human breast cancer cell line untreated (-) or treated (+) with 100 nM synthetic progestin promegestone (R5020) for 60 minutes. PVDF Membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-Progesterone R/NR3C3 (S294) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5955) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-Progesterone RB (S294) at approximately XXX kDa and Phospho-Progesterone RA (S130) at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX. |
