| 货号 | AF2990 |
| 别名 | c-Jun N-terminal kinase kinase 1; dual specificity mitogen-activated protein kinase kinase 4; JNK activating kinase 1; JNK-activated kinase 1; JNK-activating kinase 1; JNKK; JNKK1; JNKK1MAPK/ERK kinase 4; MAP kinase kinase 4; MAP2K4; MAPKK4; MEK4; MEK4MEK 4; mitogen-activated protein kinase kinase 4; MKK4SAPK/ERK kinase 1; PRKMK4; PRKMK4MAPKK 4; SEK1; SERK1; SERK1EC 2.7.12.2 | 全称 | Mitogen-activated Protein Kinase Kinase 4 |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat MKK4 when dually phosphorylated at sites corresponding to S257 and T261 of human MKK4. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6416 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide containing human MKK4 S257/T261 site |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | MKK4, also known as MEK4, JNKK1, SEK1, MAP2K4, is an intracellular protein kinase that functions in the JNK (Jun N-terminal kinase) signal transduction pathway. In response to activation by one of several MAPKKKs, MKK4 (and MKK7) phosphorylates and activates the JNKs that regulate neural development, apoptosis, inflammation, and cellular responses to stress. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Rat and Human Phospho-MKK4 (S257/T261) by Western Blot. Western blot shows lysates of PC‑12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 300 mM sorbitol for 30 minutes and HepG2 human hepatocellular carcinoma cell line untreated or treated with 10 ng/mL Recombinant Human IL-1 beta (Catalog #201-LB) for 20 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-MKK4 (S257/T261) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2990), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MKK4 (S257/T261) at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
