Phospho-RSK1 (S221)/RSK2 (S227) Affinity Purified Pab (100 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Phospho-RSK1 (S221)/RSK2 (S227) Affinity Purified Pab (100 UG)

货号： AF892 基本售价： 4094.7 元规格： -

产品信息

概述
货号	AF892
别名	RSK1/RSK2
全称	Ribosomal Protein S6 Kinase II
应用	Western Blot(0.5 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human, mouse, and rat RSK1 when phosphorylated at S221, and human and mouse RSK2 when phosphorylated at S227.
使用方法	Western Blot: 0.5 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. ERK and RSK regulate distinct steps of a cellular program that induces transition from multicellular epithelium to single cell phenotype. Authors: Caslavsky J, Klimova Z, Vomastek T Cell Signal, 2013;25(12):2743-51. Species: Canine Sample Type: whole cells Application: WB Molecular pharmacology and antitumor activity of PHT-427, a novel Akt/phosphatidylinositide-dependent protein kinase 1 pleckstrin homology domain inhibitor. Authors: Meuillet EJ, Zuohe S, Lemos R, Ihle N, Kingston J, Watkins R, Moses SA, Zhang S, Du-Cuny L, Herbst R, Jacoby JJ, Zhou LL, Ahad AM, Mash EA, Kirkpatrick DL, Powis G Mol. Cancer Ther., 2010;9(3):706-17. Species: Human Sample Type: Cell Lysates Application: WB Yersinia virulence factor YopM induces sustained RSK activation by interfering with dephosphorylation. Authors: Hentschke M, Berneking L, Belmar Campos C PLoS ONE, 2010;5(10):. Species: Human Sample Type: Cell Lysates Application: WB Ca2+ -stimulated adenylyl cyclases regulate ERK-dependent activation of MSK1 during fear conditioning. Authors: Sindreu CB, Scheiner ZS, Storm DR Neuron, 2007;53(1):79-89. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen
纯化方式	Antigen Affinity-purified
免疫原	Phosphopeptide containing human RSK1 S221 site
生物活性	Human, Mouse, Rat
标记	Unconjugated
背景	The 90 kDa ribosomal protein S6 kinases (RSK1-4) are a family of broadly expressed Ser/Thr kinases activated in response to mitogenic stimuli, including growth factors and tumor-promoting phorbol esters. RSKs are directly phosphorylated by ERK1 and ERK2, leading to RSK autophosphorylation and activation. Active RSKs appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB.
运输条件	Dry Ice
存放说明	-20℃
参考文献	Apoptosis Intracellular Kinases Chemokine Receptor Intracellular Signaling ICC / IHC Images: RSK1/RSK2 Intracellular Kinases Technical Notes: RSK1, phosphorylation at S380 Translation Regulation

参考图片

RSK1/RSK2 in Mouse Brain. RSK1/RSK2 was detected in perfusion fixed frozen sections of mouse brain (Purkinje cells in cerebellum) using 15 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF892) overnight at 4 °C. Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Human Phospho-RSK1 (S221) and RSK2 (S227) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF892), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RSK1 (S221) and RSK2 (S227) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.