| 货号 | AF1518 |
| 别名 | 90 kDa ribosomal protein S6 kinase 3; CLS; Coffin-Lowry syndrome; EC 2.7.11; EC 2.7.11.1; HU-3; Insulin-stimulated protein kinase 1; ISPK1; ISPK-1; MAP kinase-activated protein kinase 1b; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK1B; MAPKAPK-1b; mental retardation, X-linked 19; MRX19; p90-RSK2; p90RSK3; pp90RSK2; ribosomal protein S6 kinase alpha-3; ribosomal protein S6 kinase, 90kD, polypeptide 3; ribosomal protein S6 kinase, 90kDa, polypeptide 3; Ribosomal S6 kinase 2; RPS6KA3; RSK; RSK-2; RSK2p90-RSK 3; S6K-alpha3; S6K-alpha-3 | 全称 | Ribosomal Protein S6 Kinase II |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat RSK2 in Western blots. The antibody does not detect recombinant human RSK1 in Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6197 (Human); 110651 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human RSK2 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The 90 kDa ribosomal protein S6 kinases (RSK1-4) are a family of broadly expressed Ser/Thr kinases activated in response to mitogenic stimuli, including growth factors and tumor-promoting phorbol esters. RSKs are directly phosphorylated by ERK1 and ERK2, leading to RSK autophosphorylation and activation. Active RSKs appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat RSK2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, PC‑12 rat adrenal pheochromocytoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat RSK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1518) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for RSK2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
RSK2 in Mouse Embryo. RSK2 was detected in immersion fixed frozen sections of mouse embryo using Rabbit Anti-Human/Mouse/Rat RSK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1518) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
