| 货号 | AF3285 |
| 别名 | FGF R1-4 | 全称 | Fibroblast Growth Factor Receptors 1-4 |
| 应用 | Western Blot(0.5 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human FGF R when dually phosphorylated at Y653/Y654 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. FGFR2-amplified gastric cancer cell lines require FGFR2 and Erbb3 signaling for growth and survival. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide containing human FGF R1 Y653/Y654 sites |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The fibroblast growth factor receptors (FGF R), including FGF R1, R2, R3, and R4, are involved in development processes, angiogenesis, wound healing, and tumorigenesis. FGF binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Tyr653 and Tyr654 in FGF R1 and the corresponding tyrosine residues in FGF R2, R3 and R4 are important for the activation of intrinsic kinase activity. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human Phospho-FGF R1‑4 (Y653/Y654) by Western Blot. Western blot shows lysates of KATO‑III human gastric carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human FGF acidic (Catalog #232‑FA) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-FGF R1‑4 (Y653/Y654) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3285), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-FGF R1‑4 (Y653/Y654) at approximately 120 to 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Phospho-FGF R1-4 (Y653/Y654) in A431 Human Cell Line. FGF R1‑4 phosphorylated at Y653/Y654 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human Phospho-FGF R1‑4 (Y653/Y654) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3285) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
