概述| 货号 | AF2316 |
| 别名 | beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide |
全称 | Platelet-derived Growth Factor Receptor beta | | 应用 | Western Blot(0.5 µg/mL)
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| 目标/特异性 | Detects human PDGF R beta when phosphorylated at Y1021 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL
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| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 5159 (Human); 18596 (Mouse); 24629 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide containing human PDGF R beta Y1021 site |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin‑like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero‑dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.
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参考图片Detection of Human Phospho-PDGF R beta (Y1021) by Western Blot. Western blot shows lysates of human foreskin fibroblast untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF‑AA, PDGF‑AB, and PDGF‑BB (Catalog # 221-AA, 222-AB, and 220-BB, respectively) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho‑PDGF R beta (Y1021) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2316), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho‑PDGF R beta (Y1021) at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
