Phospho-Histone H2AX (S139) Affinity Purified Polyclonal Ab (50 UG)【科瑞奥博】

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Phospho-Histone H2AX (S139) Affinity Purified Polyclonal Ab (50 UG)

货号： AF2288 基本售价： 4025.3 元规格： -

产品信息

概述
货号	AF2288
别名	H2AFX
应用	Western Blot(0.5 µg/mL) Simple Western(5 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human Histone H2AX when phosphorylated at S139.
使用方法	Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3014 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Synergistic Activity of Deguelin and Fludarabine in Cells from Chronic Lymphocytic Leukemia Patients and in the New Zealand Black Murine Model Authors: N Rebolleda, I Losada-Fer, G Perez-Chac, R Castejon, S Rosado, M Morado, MT Vallejo-Cr, A Martinez, JA Vargas-Nuñ, P Perez-Acie PLoS ONE, 2016;11(4):e0154159. Species: Human Sample Type: Cell Lysates Application: WB Inhibition of topoisomerase II by 8-chloro-adenosine triphosphate induces DNA double-stranded breaks in 8-chloro-adenosine-exposed human myelocytic leukemia K562 cells. Authors: Yang SY, Jia XZ, Feng LY, Li SY, An GS, Ni JH, Jia HT Biochem. Pharmacol., 2009;77(3):433-43. Species: Human Sample Type: Cell Lysates Application: WB Inhibition of CHK1 kinase by Go6976 converts 8-chloro-adenosine-induced G2/M arrest into S arrest in human myelocytic leukemia K562 cells. Authors: Jia XZ, Yang SY, Zhou J, Li SY, Ni JH, An GS, Jia HT Biochem. Pharmacol., 2009;77(5):770-80. Species: Human Sample Type: Cell Lysates Application: WB DNA cross-linking, double-strand breaks, and apoptosis in corneal endothelial cells after a single exposure to mitomycin C. Authors: Roh DS, Cook AL, Rhee SS, Joshi A, Kowalski R, Dhaliwal DK, Funderburgh JL Invest. Ophthalmol. Vis. Sci., 2008;49(11):4837-43. Species: Goat Sample Type: Whole Cells Application: ICC Induction of MHC class I-related chain B (MICB) by 5-aza-2-deoxycytidine. Authors: Tang KF, He CX, Zeng GL, Wu J, Song GB, Shi YS, Zhang WG, Huang AL, Steinle A, Ren H Biochem. Biophys. Res. Commun., 2008;370(4):578-83. Species: Human Sample Type: Whole Cells Application: ICC Histone H2AX and Fanconi anemia FANCD2 function in the same pathway to maintain chromosome stability. Authors: Bogliolo M, Lyakhovich A, Callen E, Castella M, Cappelli E, Ramirez MJ, Creus A, Marcos R, Kalb R, Neveling K, Schindler D, Surralles J EMBO J., 2007;26(5):1340-51. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen and protein A Affinity-purified
免疫原	Phosphopeptide containing human Histone H2AX S139 site
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Histone H2AX is phosphorylated at S139 in cells exposed to DNA double-strand break-inducing agents, such as ionizing radiation. The S139 phosphorylated H2AX, termed ( gamma -H2AX, marks the site of DNA double-strand breaks and serves to recruit cell cycle checkpoint and DNA repair factors to the site of damage.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Checkpoint DNA Damage and Repair Histone Deacetylases (HDACs) and Associated Proteins ICC / IHC Images: Histone H2AX Stem Cell Epigenetics

参考图片

Histone H2AX in Human Breast Cancer Tissue. Histone H2AX was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human Phospho-Histone H2AX (S139) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2288) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Phospho-Histone H2AX (S139) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 μM camptothecin (CPT) for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-Histone H2AX (S139) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2288), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Histone H2AX (S139) at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Phospho-Histone H2AX (S139) by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 24 hours, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Histone H2AX (S139) at approximately 25 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-Histone H2AX (S139) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2288). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Phospho-Histone H2AX (S139) in HeLa Human Cell Line. Histone H2AX phosphorylated at S139 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with ultraviolet radiation using Rabbit Anti-Human Phospho-Histone H2AX (S139) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2288) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.