Human ERK1 Affinity Purified Polyclonal Ab (50 UG)【科瑞奥博】

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Human ERK1 Affinity Purified Polyclonal Ab (50 UG)

货号： AF1940 基本售价： 4025.3 元规格： -

产品信息

概述
货号	AF1940
别名	EC 2.7.11; ERK-1; ERK1p44-MAPK; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK3; MGC20180; Microtubule-associated protein 2 kinase; Mitogen-activated protein kinase 1; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; p44mapk; PRKM3; PRKM3EC 2.7.11.24
全称	Extracellular Signal-regulated Kinase 1
反应种属	Human
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects human ERK1 in Western blots. Because the peptide immunogen corresponds to a region of ERK1 largely absent in ERK2, no reactivity with ERK2 is present.
使用方法	Western Blot: 0.1 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5595 (Human); 26417 (Mouse); 50689 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Human ERK1 synthetic peptide Met1-Val28 Accession # P27361
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	ERK1 is a protein Serine/Threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain, and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Airway Inflammation and Remodeling Apoptosis Intracellular Kinases Cancer Biomarkers Chemokine Receptor Intracellular Signaling Common gamma Chain Receptor Family C-type Lectin Receptors EMT Induction GDNF Family IL-1 Family IL-17 Family Intracellular Kinases Intracellular Kinases in the Akt Pathway Intracellular Signaling Molecules in Angiogenesis Mini-review: The ERK Signal Transduction Pathway Natural Killer T (NKT) Cells Neurotrophin/Trk Family PDGF Family Positive Regulators of the Jak/STAT Pathway ROS and Stress Resistance TNF Superfamily Regulators VEGF Family

参考图片

Detection of Human ERK1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL Rabbit Anti-Human ERK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1940) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human Active ERK1 (Catalog # 1879-KS) and Recombinant Human Active ERK2 (Catalog # 1230-KS) (2 ng/lane) were included. A specific band for ERK1 was detected at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.