Human/Mouse Bax Affinity Purified Polyclonal Ab (30 UG)【科瑞奥博】

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Human/Mouse Bax Affinity Purified Polyclonal Ab (30 UG)

货号： AF820 基本售价： 4025.3 元规格： -

产品信息

概述
货号	AF820
别名	apoptosis regulator BAX; BCL2-associated X protein; Bcl2-L-4; BCL2L4bcl2-L-4; Bcl-2-like protein 4
全称	Bcl Associated X Protein
反应种属	Human/Mouse
应用	Western Blot(0.3 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human and mouse Bax.
使用方法	Western Blot: 0.3 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	581 (Human); 12028 (Mouse); 24887 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Bcl-xL Is Essential for the Survival and Function of Differentiated Neurons in the Cortex That Control Complex Behaviors J Neurosci, 2016;36(20):5448-61. Species: Mouse Sample Type: Whole Tissue Application: IHC - Paraffin embedded Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction. Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S Gut, 2006;56(2):243-52. Species: Human Sample Type: Cell Lysates Application: WB Sustained early growth response gene 3 expression inhibits the survival of CD4/CD8 double-positive thymocytes. Authors: Xi H, Kersh GJ J. Immunol., 2004;173(1):340-8. Species: Rat Sample Type: Cell Lysates Application: WB Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex. Authors: Jayanthi S, Deng X, Bordelon M, McCoy MT, Cadet JL FASEB J., 2001;15(10):1745-52. Species: Mouse Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	KLH-coupled human Bax synthetic peptide GPTSSEQIMKTGALLLQGFIQDC
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The sequence used for immunization differs significantly from the sequences of the corresponding region of other members of the Bcl-2 family.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Bax Relocates from the Cytosol to Mitochondria to Trigger Apoptosis Bcl-2 Proteins and Regulators Cell Structure and Signaling Molecules ICC / IHC Images: Bax

参考图片

Bax in Human Liver. Bax was detected in immersion fixed paraffin-embedded sections of human liver using 10 µg/mL Rabbit Anti-Human/Mouse Bax Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF820) overnight at 4 °C. Tissue was stained with the Anti-Rabbit HRP‑DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human/Mouse Bax by Western Blot. Western blot shows lysates of CHP‑100 human neuroblastoma cell line, THP‑1 human acute monocytic leukemia cell line, L‑929 mouse fibroblast cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.3 µg/mL of Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bax at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Bax in Human Breast. Bax was detected in immersion fixed paraffin-embedded sections of human breast using 15 µg/mL Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.