Human Survivin Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human Survivin Affinity Purified Polyclonal Ab (100 UG)

货号： AF886 基本售价： 4025.3 元规格： -

产品信息

概述
货号	AF886
别名	API4 survivin variant 3 alpha; API4; Apoptosis inhibitor 4 baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; BIRC5; EPR-1; IAP4
反应种属	Human
应用	Western Blot(0.5 µg/mL) Simple Western(5 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human Survivin. This antibody also detects an unidentified ~51 kDa protein band.
使用方法	Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	332 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma Authors: Katharina Engel EMBO Mol Med, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: WB Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies Molecules, 2016;21(8):. Species: Human Sample Type: Whole Cells Application: Flow Delivery of a survivin promoter-driven antisense survivin-expressing plasmid DNA as a cancer therapeutic: a proof-of-concept study Onco Targets Ther, 2016;9(0):2601-13. Species: Human Sample Type: Cell Lysates Application: WB STAT3 activation is required for the antiapoptotic effects of prolactin in cervical cancer cells. Authors: Ramirez de Arellano A, Lopez-Pulido E, Martinez-Neri P, Estrada Chavez C, Gonzalez Lucano R, Fafutis-Morris M, Aguilar-Lemarroy A, Munoz-Valle J, Pereira-Suarez A Cancer Cell Int, 2015;15(0):83. Species: Human Sample Type: Cell Lysates Application: WB Sulindac sulfide inhibits colon cancer cell growth and downregulates specificity protein transcription factors. Authors: Li X, Pathi S, Safe S BMC Cancer, 2015;15(0):974. Species: Human Sample Type: Cell Lysates Application: WB Survivin expression promotes VEGF-induced tumor angiogenesis via PI3K/Akt enhanced beta-catenin/Tcf-Lef dependent transcription. Authors: Fernandez J, Rodriguez D, Valenzuela M, Calderon C, Urzua U, Munroe D, Rosas C, Lemus D, Diaz N, Wright M, Leyton L, Tapia J, Quest A Mol Cancer, 2014;13(0):209. Species: Human Sample Type: Cell Lysates Application: WB COX-2-independent effects of celecoxib sensitize lymphoma B cells to TRAIL-mediated apoptosis. Authors: Gallouet A, Travert M, Bresson-Bepoldin L, Guilloton F, Pangault C, Caulet-Maugendre S, Lamy T, Tarte K, Guillaudeux T Clin Cancer Res, 2014;20(10):2663-73. Species: Human Sample Type: Cell Lysates Application: WB The pterocarpanquinone LQB-118 induces apoptosis in acute myeloid leukemia cells of distinct molecular subtypes and targets FoxO3a and FoxM1 transcription factors. Authors: Nestal de Moraes G, Castro C, Salustiano E, Dumas M, Costas F, Lam E, Costa P, Maia R Int J Oncol, 2014;45(5):1949-58. Species: Human Sample Type: Total protein Application: WB Sequence-dependent combination therapy with doxorubicin and a survivin-specific small interfering RNA nanodrug demonstrates efficacy in models of adenocarcinoma. Authors: Ghosh S, Yigit M, Uchida M, Ross A, Barteneva N, Moore A, Medarova Z Int J Cancer, 2014;134(7):1758-66. Species: Human Sample Type: Cell Lysates Application: WB Protein kinase C zeta regulates survivin expression and inhibits apoptosis in colon cancer. Authors: Umemori Y, Kuribayashi K, Nirasawa S, Kondoh T, Tanaka M, Kobayashi D, Watanabe N Int J Oncol, 2014;45(3):1043-50. Species: Human Sample Type: Total protein Application: WB Azadirone, a limonoid tetranortriterpene, induces death receptors and sensitizes human cancer cells to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) through a p53 protein-independent mechanism: evidence for the role of the ROS-ERK-CHOP-death receptor pathway. Authors: Gupta, Subash C, Francis, Sajin K, Nair, Mangalam, Mo, Yin-Yuan, Aggarwal, Bharat B J Biol Chem, 2013;288(45):32343-56. Species: Human Sample Type: Cell Lysates Application: WB The novel c-Met inhibitor cabozantinib overcomes gemcitabine resistance and stem cell signaling in pancreatic cancer. Authors: Hage C, Rausch V, Giese N, Giese T, Schonsiegel F, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Herr I Cell Death Dis, 2013;4(0):e627. Species: Human Sample Type: Cell Lysates Application: WB Phenotypic characterization of hereditary epithelial ovarian cancer based on a tissue microarray study. Authors: Munoz-Repeto I, Garcia M, Kamieniak M, Ramon Y Cajal T, Domingo S, Cazorla A, Garcia Donas J, Hernando Polo S, Garcia Sagredo J, Hernandez E, Lacambra C, Saez R, Robles L, Borrego S, Prat J, Palacios J, Benitez J Histol Histopathol, 2013;28(1):133-44. Species: Human Sample Type: whole cells Application: IHC Paraffin-embedded Dacarbazine and the agonistic TRAIL receptor-2 antibody lexatumumab induce synergistic anticancer effects in melanoma. Authors: Engesaeter B, Engebraaten O, Florenes V, Maelandsmo G PLoS ONE, 2012;7(9):e45492. Species: Human Sample Type: Cell Lysates Application: WB RIP1 is required for IAP inhibitor-mediated sensitization of childhood acute leukemia cells to chemotherapy-induced apoptosis. Authors: Loder S, Fakler M, Schoeneberger H, Cristofanon S, Leibacher J, Vanlangenakker N, Bertrand MJ, Vandenabeele P, Jeremias I, Debatin KM, Fulda S Leukemia, 2011;26(5):1020-9. Species: Human Sample Type: Cell Lysates Application: WB Serum levels of inhibitors of apoptotic proteins (IAPs) change with IVIg therapy in pemphigus. Authors: Toosi, Siavash, Habib, Nancy, Torres, Geneviev, Reynolds, Sandra R, Bystryn, Jean-Cla J Invest Dermatol, 2011;131(11):2327-9. Species: Human Sample Type: Serum Application: WB Myricetin sensitizes malignant glioma cells to TRAIL-mediated apoptosis by down-regulation of the short isoform of FLIP and bcl-2. Authors: Siegelin MD, Gaiser T, Habel A, Siegelin Y Cancer Lett., 2009;283(2):230-8. Species: Human Sample Type: Whole Cells Application: WB SOX7, down-regulated in colorectal cancer, induces apoptosis and inhibits proliferation of colorectal cancer cells. Authors: Zhang Y, Huang S, Dong W, Li L, Feng Y, Pan L, Han Z, Wang X, Ren G, Su D, Huang B, Lu J Cancer Lett., 2009;277(1):29-37. Species: Human Sample Type: Cell Lysates Application: WB Regulation of apoptosis by XIAP ubiquitin-ligase activity. Authors: Schile AJ, Garcia-Fernandez M, Steller H Genes Dev., 2008;22(16):2256-66. Species: Mouse Sample Type: Cell Lysates Application: WB Cell polarization during monopolar cytokinesis. Authors: Hu CK, Coughlin M, Field CM, Mitchison TJ J. Cell Biol., 2008;181(2):195-202. Species: Human Sample Type: Whole Cells Application: ICC Survivin in the human hair follicle. Authors: Botchkareva NV, Kahn M, Ahluwalia G, Shander D J. Invest. Dermatol., 2007;127(2):479-82. Species: Human Sample Type: Whole Tissue Application: IHC Nuclear localization of Survivin renders HeLa tumor cells more sensitive to apoptosis by induction of p53 and Bax. Authors: Temme A, Rodriguez JA, Hendruschk S, Gunes S, Weigle B, Schakel K, Schmitz M, Bachmann M, Schackert G, Rieber EP Cancer Lett., 2007;250(2):177-93. Species: Human Sample Type: Whole Cells Application: ICC Mitogen-activated protein kinase kinase inhibition enhances nuclear proapoptotic function of p53 in acute myelogenous leukemia cells. Authors: Kojima K, Konopleva M, Samudio IJ Cancer Res., 2007;67(7):3210-9. Species: Human Sample Type: Cell Lysates Application: WB cIAP-2 and survivin contribute to cytokine-mediated delayed eosinophil apoptosis. Authors: Vassina EM, Yousefi S, Simon D, Zwicky C, Conus S, Simon HU Eur. J. Immunol., 2006;36(7):1975-84. Species: Human Sample Type: Cell Lysates Application: WB Indirubin-3'-monoxime, a CDK inhibitor induces growth inhibition and apoptosis-independent up-regulation of survivin in transitional cell cancer. Authors: Perabo FG, Frossler C, Landwehrs G, Schmidt DH, von Rucker A, Wirger A, Muller SC Anticancer Res., 2006;26(3):2129-35. Species: Human Sample Type: Cell Lysates Application: WB Survivin interacts with Smac/DIABLO in ovarian carcinoma cells but is redundant in Smac-mediated apoptosis. Authors: McNeish IA, Lopes R, Bell SJ, McKay TR, Fernandez M, Lockley M, Wheatley SP, Lemoine NR Exp. Cell Res., 2005;302(1):69-82. Species: Human Sample Type: Cell Lysates Application: Affinity Chromatography Apoptotic signaling pathways induced by nitric oxide in human lymphoblastoid cells expressing wild-type or mutant p53. Authors: Li CQ, Robles AI, Hanigan CL, Hofseth LJ, Trudel LJ, Harris CC, Wogan GN Cancer Res., 2004;64(9):3022-9. Species: Human Sample Type: Cell Lysates Application: WB Inflammation-associated cell cycle-independent block of apoptosis by survivin in terminally differentiated neutrophils. Authors: Altznauer F, Martinelli S, Yousefi S, Thurig C, Schmid I, Conway EM, Schoni MH, Vogt P, Mueller C, Fey MF, Zangemeister-Wittke U, Simon HU J. Exp. Med., 2004;199(10):1343-54. Species: Human Sample Type: Whole Cells Application: WB The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly. Authors: Sampath SC, Ohi R, Leismann O, Salic A, Pozniakovski A, Funabiki H Cell, 2004;118(2):187-202. Species: Human Sample Type: Cell Lysates Application: WB Localization, dynamics, and function of survivin revealed by expression of functional survivinDsRed fusion proteins in the living cell. Authors: Temme A, Rieger M, Reber F, Lindemann D, Weigle B, Diestelkoetter-Bachert P, Ehninger G, Tatsuka M, Terada Y, Rieber EP Mol. Biol. Cell, 2003;14(1):78-92. Species: Human Sample Type: Whole Cells Application: ICC Elevated expression level of survivin protein in soft-tissue sarcomas is a strong independent predictor of survival. Authors: Kappler M, Kotzsch M, Bartel F, Fussel S, Lautenschlager C, Schmidt U, Wurl P, Bache M, Schmidt H, Taubert H, Meye A Clin. Cancer Res., 2003;9(3):1098-104. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Survivin aa 1-142
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Survivin is a member of the Inhibitor of Apoptosis (IAP) gene family and contains a single BIR domain. Increased survivin expression at the G2/M checkpoint contributes to tissue homeostasis and differentiation during embryonic and fetal development. Increased expression in differentiated adult tissues, however, frequently results in unchecked cell survival, resulting in cancer. Survivin can protect cells from caspase-induced apoptosis activated in mitosis presumably by binding to Caspase-3, -7, and -9.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Cancer Biomarkers Caspases and Regulators Cysteine Proteases and Regulators HIF Transcription Factors ICC / IHC Images: Survivin Inhibitors of Apoptosis (IAPs) and Regulators

参考图片

Survivin in Human Prostate Cancer Tissue. Survivin was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 10 µg/mL Rabbit Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF886) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Survivin by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF886) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Survivin at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Detection of Human Survivin by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Survivin at approximately 23 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF886). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.