Human/Mouse FLIP Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human/Mouse FLIP Affinity Purified Polyclonal Ab (100 UG)

货号： AF821 基本售价： 4025.3 元规格： -

产品信息

概述
货号	AF821
别名	CASHFADD-like antiapoptotic molecule 1; CASP8 and FADD-like apoptosis regulator; CASP8AP1; Caspase homolog; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; caspase-related inducer of apoptosis; CASPER; CasperFLAME1; Cellular FLICE-like inhibitory protein; CFLAR; c-FLIPR; c-FLIPS; CLARPMACH-related inducer of toxicity; FADD-like apoptotic molecule; FLAME; FLAME-1; I-FLICE; I-FLICEMRITc-FLIPFLIPc-FLIPL; Inhibitor of FLICE; usurpin beta; Usurpin
全称	FLICE Inhibitory Proteins
反应种属	Human/Mouse
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human and mouse FLIP in Western blots.
使用方法	Western Blot: 1 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8837 (Human); 12633 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Infrared radiation confers resistance to UV-induced apoptosis via reduction of DNA damage and upregulation of antiapoptotic proteins. Authors: Jantschitsch C, Majewski S, Maeda A, Schwarz T, Schwarz A J. Invest. Dermatol., 2009;129(5):1271-9. Species: Mouse Sample Type: Whole Cells Application: Flow Synergistic interactions between interferon-gamma and TRAIL modulate c-FLIP in endothelial cells, mediating their lineage-specific sensitivity to thrombotic thrombocytopenic purpura plasma-associated apoptosis. Authors: Stefanescu R, Bassett D, Modarresi R, Santiago F, Fakruddin M, Laurence J Blood, 2008;112(2):340-9. Species: Human Sample Type: Cell Lysates Application: WB Low concentration of interleukin-1beta induces FLICE-inhibitory protein-mediated beta-cell proliferation in human pancreatic islets. Authors: Maedler K, Schumann DM, Sauter N, Ellingsgaard H, Bosco D, Baertschiger R, Iwakura Y, Oberholzer J, Wollheim CB, Gauthier BR, Donath MY Diabetes, 2006;55(10):2713-22. Species: Human Sample Type: Cell Lysates Application: WB Expression of Fas but not Fas ligand on fetal pig beta cells. Authors: Bai L, Maedler K, Donath M, Tuch BE Xenotransplantation, 2004;11(5):426-35. Species: Porcine Sample Type: Whole Cells Application: ICC The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells. Authors: Sade H, Krishna S, Sarin A J. Biol. Chem., 2004;279(4):2937-44. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	KLH-coupled mouse FLIP synthetic peptide LKQGRRRPLVDLHVELMDKC
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	FLIP (Flice-like Inhibitory Protein), also known as I-FLICE, CASPER, and FLAME-1, is an apoptosis inhibitory protein with architecture similar to that of Caspases-8 and -10. Both of the FLIP isoforms, Long (L) and Short (S), contain two death effector domains (DED). FLIP(L) has a C-terminal Caspase-like protease domain which lacks both a catalytic active site and residues that form a substrate-binding pocket. FLIP(S) and FLIP(L) interact with the adaptor molecule FADD, and potently inhibit apoptosis initiated by Fas Ligand/TNF signaling pathways.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Adaptor Proteins Adaptor Proteins in the Akt Pathway Apoptosis Adaptor Proteins Caspases and Regulators Cysteine Proteases and Regulators TNF Superfamily TNF Superfamily Regulators

参考图片

Detection of Human/Mouse FLIP by Western Blot. Western blot shows lysates of WS‑1 human fetal skin fibroblast cell line and L‑929 mouse fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse FLIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF821) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for FLIP at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.