Human/Mouse/Rat HTRA2/Omi Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human/Mouse/Rat HTRA2/Omi Affinity Purified Polyclonal Ab (100 UG)

货号： AF1458 基本售价： 4025.3 元规格： -

产品信息

概述
货号	AF1458
别名	High temperature requirement protein A2; HtrA serine peptidase 2; HtrA2; HtrA-like serine protease; Omi; OMIOmi stress-regulated endoprotease; PARK13EC 3.4.21.108; PRSS25; PRSS25serine, 25; Serine protease 25; serine protease HTRA2, mitochondrial; Serine proteinase OMI
全称	High Temperature Requirement Protein-2
反应种属	Human/Mouse/Rat
应用	Western Blot(0.25 µg/mL) Simple Western(2.5 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human, mouse, and rat full length and mitochondria-processed HTRA2/Omi.
使用方法	Western Blot: 0.25 µg/mL Simple Western: 2.5 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	27429 (Human); 64704 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Protease Omi facilitates neurite outgrowth in mouse neuroblastoma N2a cells by cleaving transcription factor E2F1. Authors: Ma Q, Hu Q, Xu R, Zhen X, Wang G Acta Pharmacol Sin, 2015;36(8):966-75. Species: Human Sample Type: Cell Lysates Application: WB Protease Omi cleaving Hax-1 protein contributes to OGD/R-induced mitochondrial damage in neuroblastoma N2a cells and cerebral injury in MCAO mice. Authors: Wu J, Li M, Cao L, Sun M, Chen D, Ren H, Xia Q, Tao Z, Qin Z, Hu Q, Wang G Acta Pharmacol Sin, 2015;36(9):1043-52. Species: Mouse Sample Type: Tissue Homogenates Application: WB p53-mediated activation of the mitochondrial protease HtrA2/Omi prevents cell invasion. Authors: Yamauchi S, Hou Y, Guo A, Hirata H, Nakajima W, Yip A, Yu C, Harada I, Chiam K, Sawada Y, Tanaka N, Kawauchi K J Cell Biol, 2014;204(7):1191-207. Species: Mouse Sample Type: Whole Cells Application: IF Influenza A virus protein PB1-F2 translocates into mitochondria via Tom40 channels and impairs innate immunity. Authors: Yoshizumi T, Ichinohe T, Sasaki O, Otera H, Kawabata S, Mihara K, Koshiba T Nat Commun, 2014;5(0):4713. Species: Human Sample Type: Cell Lysates Application: WB The accumulation of misfolded proteins in the mitochondrial matrix is sensed by PINK1 to induce PARK2/Parkin-mediated mitophagy of polarized mitochondria. Authors: Jin, Seok Min, Youle, Richard Autophagy, 2013;9(11):1750-7. Species: Human Sample Type: Cell Lysates Application: WB PINK1 is degraded through the N-end rule pathway. Authors: Yamano, Koji, Youle, Richard Autophagy, 2013;9(11):1758-69. Species: Human Sample Type: Cell Lysates Application: WB PINK1/Parkin-mediated mitophagy is dependent on VDAC1 and p62/SQSTM1. Authors: Geisler S, Holmstrom KM, Skujat D, Fiesel FC, Rothfuss OC, Kahle PJ, Springer W Nat. Cell Biol., 2010;12(2):119-31. Species: Human Sample Type: Whole Cells Application: ICC Enhanced HtrA2/Omi expression in oxidative injury to retinal pigment epithelial cells and murine models of neurodegeneration. Authors: Ding X, Patel M, Shen D, Herzlich AA, Cao X, Villasmil R, Klupsch K, Tuo J, Downward J, Chan CC Invest. Ophthalmol. Vis. Sci., 2009;50(10):4957-66. Species: Human Sample Type: Whole Cells Application: ICC Novel mitochondrial substrates of omi indicate a new regulatory role in neurodegenerative disorders. Authors: Johnson F, Kaplitt MG, PLoS ONE, 2009;4(9):e7100. Species: Human Sample Type: Cell Lysates Application: WB Identification of a novel protein MICS1 that is involved in maintenance of mitochondrial morphology and apoptotic release of cytochrome c. Authors: Oka T, Sayano T, Tamai S, Yokota S, Kato H, Fujii G, Mihara K Mol. Biol. Cell, 2008;19(6):2597-608. Species: Human Sample Type: Whole Cells Application: ICC HtrA2 regulates beta-amyloid precursor protein (APP) metabolism through endoplasmic reticulum-associated degradation. Authors: Huttunen HJ, Guenette SY, Peach C, Greco C, Xia W, Kim DY, Barren C, Tanzi RE, Kovacs DM J. Biol. Chem., 2007;282(38):28285-95. Species: Hamster Sample Type: Cell Lysates Application: WB Induction of BIM(EL) following growth factor withdrawal is a key event in caspase-dependent apoptosis of 661W photoreceptor cells. Authors: Gomez-Vicente V, Doonan F, Donovan M, Cotter TG Eur. J. Neurosci., 2006;24(4):981-90. Species: Mouse Sample Type: Whole Cells Application: ICC Yersinia YopP-induced apoptotic cell death in murine dendritic cells is partially independent from action of caspases and exhibits necrosis-like features. Authors: Grobner S, Autenrieth SE, Soldanova I, Gunst DS, Schaller M, Bohn E, Muller S, Leverkus M, Wesselborg S, Autenrieth IB, Borgmann S Apoptosis, 2006;11(11):1959-68. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Role and regulation of nodal/activin receptor-like kinase 7 signaling pathway in the control of ovarian follicular atresia. Authors: Wang H, Jiang JY, Zhu C, Peng C, Tsang BK Mol. Endocrinol., 2006;20(10):2469-82. Species: Rat Sample Type: Cell Lysates Application: WB Roscovitine-induced up-regulation of p53AIP1 protein precedes the onset of apoptosis in human MCF-7 breast cancer cells. Authors: Wesierska-Gadek J, Gueorguieva M, Horky M Mol. Cancer Ther., 2005;4(1):113-24. Species: Human Sample Type: Whole Cells Application: ICC Motoneuron resistance to apoptotic cell death in vivo correlates with the ratio between X-linked inhibitor of apoptosis proteins (XIAPs) and its inhibitor, XIAP-associated factor 1. Authors: Perrelet D, Perrin FE, Liston P, Korneluk RG, MacKenzie A, Ferrer-Alcon M, Kato AC J. Neurosci., 2004;24(15):3777-85. Species: Rat Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human HTRA2/Omi Ala134-Glu458 Accession # O43464
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	HtrA2/Omi is the mammalian homologue of bacterial high temperature requirement protein (HtrA). HtrA2/Omi localizes to the mitochondria and is processed to expose an amino-terminal Reaper-like motif similar to SMAC/Diablo. HtrA2/Omi is released from the mitochondria in response to apoptotic insult and can interact with the BIR2 or BIR3 domains of XIAP to relieve caspase-IAP inhibition. This effect can be measured by reversing XIAP-BIR2 (R&D Systems, Catalog # 786-XB) inhibition of Caspase-7 (R&D Systems, Catalog # 823-C7) cleavage of a fluorogenic peptide (DEVD-AFC, MP Bio, Catalog # AFC-138). IC₅₀ values for this effect are typically between 0.2 and 1.5 μM. HtrA2/Omi is trimeric and functions as a serine protease. The serine protease activity may play a more central role in apoptosis than its IAP antagonizing function. A PDZ domain regulates the serine protease activity by blocking access to the active site. The specificity of the protease is yet to be defined and no endogenous substrates are known to date.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Suzuki, Y. et al. (2001) Mol. Cell. 8:613. van Loo, G. et al. (2002) Cell Death & Diff. 9:20. Hedge, R. et al. (2001) J. Biol. Chem. 277:432. Verhagen, A. et al. (2001) J. Biol. Chem. 277:445. Martins, L. et al. (2002) J. Biol. Chem. 277:439. Silke, J., and A. Verhagen (2002) Cell Death & Diff.9:362. Savopoulos, J. et al. (2000) Protein Expression & Purification 19:227.

参考图片

Detection of Human/Mouse/Rat HTRA2/Omi by Western Blot. Western blot shows lysates of PC‑12 rat adrenal pheochromocytoma cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, L‑929 mouse fibroblast cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human/Mouse/Rat HTRA2/Omi Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1458) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for HTRA2/Omi at approximately 36 and 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

HTRA2/Omi in Jurkat Human Cell Line. HTRA2/Omi was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with staurosporin using Rabbit Anti-Human/Mouse/Rat HTRA2/Omi Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1458) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (yellow; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human and Mouse HTRA2/Omi by Simple Western^TM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for HTRA2/Omi at approximately 51 kDa (precursor) and 41 kDa (processed) (as indicated) using 2.5 µg/mL of Rabbit Anti-Human/Mouse/Rat HTRA2/Omi Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1458). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.