| 货号 | BBA18 |
| 别名 | CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E |
| 反应种属 | Human |
| 应用 | Western Blot(1:1000 dilution) Immunohistochemistry(1:100 dilution) |
| 目标/特异性 | This antibody has been screened using CHO cells transfected with cDNAs for E-Selectin, P-Selectin, L-Selectin, ICAM-1 and VCAM‑1. This antibody has shown to be only reactive with E-Selectin. |
| 使用方法 | Western Blot: 1:1000 dilution Immunohistochemistry: 1:100 dilution |
| 来源 | Reconstitute in 0.5 mL of sterile water. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6401 (Human); 20339 (Mouse); 25544 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes. | |
| 纯化方式 | N/A |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human E-Selectin/CD62E |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute in 0.5 mL of sterile water. |
| 背景 | E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouse E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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E‑Selectin/CD62E in Human Kidney Cancer Tissue. E‑Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human E‑Selectin/ CD62E Polyclonal Antibody (Catalog # BBA18) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
