Human Nanog Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Nanog Affinity Purified Polyclonal Ab (25 UG)

货号： AF1997-SP 基本售价： 2488.5 元规格： -

产品信息

概述
货号	AF1997-SP
别名	FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox
全称	Nanog Homeobox
反应种属	Human
应用	Western Blot(1 µg/mL) Chromatin Immunoprecipitation (ChIP)(5 µg/5 x 10⁶cells) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human Nanog in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Nanog is observed.
使用方法	Western Blot: 1 µg/mL Chromatin Immunoprecipitation (ChIP): 5 µg/5 x 10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	79923 (Human); 71950 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity Authors: O Guillevic, S Ferratge, J Pascaud, C Driancourt, J Boyer-Di-P, G Uzan PLoS ONE, 2016;11(4):e0152993. Species: Human Sample Type: Whole Cells Application: IHC Fresh Characterisation of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming Authors: C Eguizabal Stem Cells, 2016;0(0):. Species: Human Sample Type: Whole Tissue Application: IHC - Paraffin embedded A deleterious Nav1.1 mutation selectively impairs telencephalic inhibitory neurons derived from Dravet Syndrome patients Elife, 2016;5(0):. Species: Human Sample Type: Whole Cells Application: ICC Dynamic regulation of the cancer stem cell compartment by Cripto-1 in colorectal cancer. Authors: Francescangeli F, Contavalli P, De Angelis M, Baiocchi M, Gambara G, Pagliuca A, Fiorenzano A, Prezioso C, Boe A, Todaro M, Stassi G, Castro N, Watanabe K, Salomon D, De Maria R, Minchiotti G, Zeuner A Cell Death Differ, 2015;22(10):1700-13. Species: Human Sample Type: Whole Cells Application: Flow Metabolic rescue in pluripotent cells from patients with mtDNA disease. Authors: Ma H, Folmes C, Wu J, Morey R, Mora-Castilla S, Ocampo A, Ma L, Poulton J, Wang X, Ahmed R, Kang E, Lee Y, Hayama T, Li Y, Van Dyken C, Gutierrez N, Tippner-Hedges R, Koski A, Mitalipov N, Amato P, Wolf D, Huang T, Terzic A, Laurent L, Izpisua Belmonte J, Mitalipov S Nature, 2015;524(7564):234-8. Species: Human Sample Type: Whole Cells Application: IHC - Not specified beta-Catenin and NF-kappaB co-activation triggered by TLR3 stimulation facilitates stem cell-like phenotypes in breast cancer. Authors: Jia D, Yang W, Li L, Liu H, Tan Y, Ooi S, Chi L, Filion L, Figeys D, Wang L Cell Death Differ, 2015;22(2):298-310. Species: Human Sample Type: Cell Lysates Application: WB Induced overexpression of Oct4A in human dental pulp cells enhances pluripotency and multilineage differentiation capability. Authors: Liu L, Wu L, Wei X, Ling J Stem Cells Dev, 2015;24(8):962-72. Species: Human Sample Type: Whole Cells Application: ICC Fluorescence GGGGCC repeat expansion in C9orf72 compromises nucleocytoplasmic transport. Authors: Freibaum B, Lu Y, Lopez-Gonzalez R, Kim N, Almeida S, Lee K, Badders N, Valentine M, Miller B, Wong P, Petrucelli L, Kim H, Gao F, Taylor J Nature, 2015;525(7567):129-33. Species: Human Sample Type: Whole Cells Application: ICC Morphological differences of primary cilia between human induced pluripotent stem cells and their parental somatic cells. Authors: Nathwani B, Miller C, Yang T, Solimano J, Liao J Stem Cells Dev, 2014;23(2):115-23. Species: Human Sample Type: whole cells Application: ICC Fluorescence Characterization and molecular profiling of PSEN1 familial Alzheimer's disease iPSC-derived neural progenitors. Authors: Sproul A, Jacob S, Pre D, Kim S, Nestor M, Navarro-Sobrino M, Santa-Maria I, Zimmer M, Aubry S, Steele J, Kahler D, Dranovsky A, Arancio O, Crary J, Gandy S, Noggle S PLoS ONE, 2014;9(1):e84547. Species: Human Sample Type: Whole Cells Application: ICC Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells. Authors: Luo, Lan, Kawakatsu, Miho, Guo, Chao-Wan, Urata, Yoshishi, Huang, Wen-Jing, Ali, Haytham, Doi, Hanako, Kitajima, Yuriko, Tanaka, Takayuki, Goto, Shinji, Ono, Yusuke, Xin, Hong-Bo, Hamano, Kimikazu, Li, Tao-Shen Sci Rep, 2014;4(0):3779. Species: Human Sample Type: Cell Lysate Application: WB Selective lentiviral gene delivery to CD133-expressing human glioblastoma stem cells. Authors: Bayin, N Sumru, Modrek, Aram S, Dietrich, August, Lebowitz, Jonathan, Abel, Tobias, Song, Hae-Ri, Schober, Markus, Zagzag, David, Buchholz, Christia, Chao, Moses V, Placantonakis, Dimitris PLoS ONE, 2014;9(12):e116114. Species: Human Sample Type: Whole Cells Application: ICC Fluorescence A multiplex high-throughput gene expression assay to simultaneously detect disease and functional markers in induced pluripotent stem cell-derived retinal pigment epithelium. Authors: Ferrer M, Corneo B, Davis J, Wan Q, Miyagishima K, King R, Maminishkis A, Marugan J, Sharma R, Shure M, Temple S, Miller S, Bharti K Stem Cells Transl Med, 2014;3(8):911-22. Species: Human Sample Type: Whole Cells Application: IHC Fresh Influence of activin A supplementation during human embryonic stem cell derivation on germ cell differentiation potential. Authors: Duggal G, Heindryckx B, Warrier S, OLeary T, Van der Jeught M, Lierman S, Vossaert L, Deroo T, Deforce D, Chuva de Sousa Lopes S, De Sutter P Stem Cells Dev, 2013;22(23):3141-55. Species: Human Sample Type: whole cells Application: ICC Efficient generation of human iPSCs by a synthetic self-replicative RNA. Authors: Yoshioka N, Gros E, Li H, Kumar S, Deacon D, Maron C, Muotri A, Chi N, Fu X, Yu B, Dowdy S Cell Stem Cell, 2013;13(2):246-54. Species: Human Sample Type: Whole Cells Application: IF Isogenic human pluripotent stem cell pairs reveal the role of a KCNH2 mutation in long-QT syndrome. Authors: Bellin, Milena, Casini, Simona, Davis, Richard, DAniello, Cristina, Haas, Jessica, Ward-van Oostwaard, Dorien, Tertoolen, Leon G J, Jung, Christia, Elliott, David A, Welling, Andrea, Laugwitz, Karl-Lud, Moretti, Alessand, Mummery, Christin EMBO J, 2013;32(24):3161-75. Species: Human Sample Type: Whole Cells Application: ICC Differential L1 regulation in pluripotent stem cells of humans and apes. Authors: Marchetto M, Narvaiza I, Denli A, Benner C, Lazzarini T, Nathanson J, Paquola A, Desai K, Herai R, Weitzman M, Yeo G, Muotri A, Gage F Nature, 2013;503(7477):525-9. Species: bonobo, primate, P. paniscus Sample Type: whole cells Application: ICC Sox2 expression in breast tumours and activation in breast cancer stem cells. Authors: Leis O, Eguiara A, Lopez-Arribillaga E, Alberdi MJ, Hernandez-Garcia S, Elorriaga K, Pandiella A, Rezola R, Martin AG Oncogene, 2012;31(11):1354-65. Species: Human Sample Type: Whole Tissue Application: IHC Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium. Authors: Hayashi, Ryuhei, Ishikawa, Yuki, Ito, Miyuki, Kageyama, Tomofumi, Takashiba, Kuniko, Fujioka, Tsuyoshi, Tsujikawa, Motokazu, Miyoshi, Hiroyuki, Yamato, Masayuki, Nakamura, Yukio, Nishida, Kohji PLoS ONE, 2012;7(9):e45435. Species: Human Sample Type: Whole Cells Application: ICC The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cells. Authors: Pekkanen-Mattila M, Ojala M, Kerkela E, Rajala K, Skottman H, Aalto-Setala K Stem Cells Int, 2012;2012(0):875059. Species: Human Sample Type: Whole Cells Application: ICC Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes. Authors: Emdad L, DSouza SL, Kothari HP, Qadeer ZA, Germano IM Stem Cells Dev., 2012;21(3):404-10. Species: Human Sample Type: Whole Cells Application: ICC Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells. Authors: Kim SY, Kim MJ, Jung H Stem Cells Dev., 2012;21(8):1272-86. Species: Human Sample Type: Whole Cells Application: ICC CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells. Authors: Jiang W, Sui X, Zhang D, Liu M, Ding M, Shi Y, Deng H Stem Cells, 2011;29(4):609-17. Species: Human Sample Type: Whole Cells Application: ICC A novel role for an RNA polymerase III subunit POLR3G in regulating pluripotency in human embryonic stem cells. Authors: Wong RC, Pollan S, Fong H, Ibrahim A, Smith EL, Ho M, Laslett AL, Donovan PJ Stem Cells, 2011;29(10):1517-27. Species: Human Sample Type: Cell Lysates Application: ChIP Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome. Authors: Carvajal-Vergara X, Sevilla A, DSouza SL Nature, 2010;465(7299):808-12. Species: Human Sample Type: Whole Cells Application: ICC A defined glycosaminoglycan-binding substratum for human pluripotent stem cells. Authors: Klim JR, Li L, Wrighton PJ Nat. Methods, 2010;7(12):989-94. Species: Human Sample Type: Whole Cells Application: ICC Alginate microcapsule for propagation and directed differentiation of hESCs to definitive endoderm. Authors: Chayosumrit M, Tuch B, Sidhu K Biomaterials, 2010;31(3):505-14. Species: Human Sample Type: Cell Lysate Application: WB Activation of pluripotency genes in human fibroblast cells by a novel mRNA based approach. Authors: Plews JR, Li J, Jones M, Moore HD, Mason C, Andrews PW, Na J PLoS ONE, 2010;5(12):e14397. Species: Human Sample Type: Cell Lysates Application: WB SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells. Authors: Chng Z, Teo A, Pedersen RA, Vallier L Cell Stem Cell, 2010;6(1):59-70. Species: Human Sample Type: Cell Lysates Application: ChIP Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells. Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH FEBS Lett., 2009;583(3):561-7. Species: Human Sample Type: Whole Cells Application: ICC Automated, scalable culture of human embryonic stem cells in feeder-free conditions. Authors: Thomas RJ, Anderson D, Chandra A, Smith NM, Young LE, Williams D, Denning C Biotechnol. Bioeng., 2009;102(6):1636-44. Species: Human Sample Type: Whole Cells Application: ICC Induced pluripotent stem cells offer new approach to therapy in thalassemia and sickle cell anemia and option in prenatal diagnosis in genetic diseases. Authors: Ye L, Chang JC, Lin C, Sun X, Yu J, Kan YW Proc. Natl. Acad. Sci. U.S.A., 2009;106(24):9826-30. Species: Human Sample Type: Whole Cells Application: ICC The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used. J. Cell. Biochem., 2009;106(5):867-77. Species: Human Sample Type: Whole Cells Application: ICC Adipogenic differentiation of human induced pluripotent stem cells: comparison with that of human embryonic stem cells. Authors: Taura D, Noguchi M, Sone M, Hosoda K, Mori E, Okada Y, Takahashi K, Homma K, Oyamada N, Inuzuka M, Sonoyama T, Ebihara K, Tamura N, Itoh H, Suemori H, Nakatsuji N, Okano H, Yamanaka S, Nakao K FEBS Lett., 2009;583(6):1029-33. Species: Human Sample Type: Whole Cells Application: ICC High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells. Authors: Desbordes SC, Placantonakis DG, Ciro A, Socci ND, Lee G, Djaballah H, Studer L Cell Stem Cell, 2008;2(6):602-12. Species: Human Sample Type: Whole Cells Application: ICC Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Authors: Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S Cell, 2007;131(5):861-72. Species: Human Sample Type: Cell Lysates Application: WB Pedigreed primate embryonic stem cells express homogeneous familial gene profiles. Authors: Navara CS, Mich-Basso JD, Redinger CJ, Ben-Yehudah A, Jacoby E, Kovkarova-Naumovski E, Sukhwani M, Orwig K, Kaminski N, Castro CA, Simerly CR, Schatten G Stem Cells, 2007;25(11):2695-704. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Cells Application: ICC Isolation of a novel population of multipotent adult stem cells from human hair follicles. Authors: Yu H, Fang D, Kumar SM, Li L, Nguyen TK, Acs G, Herlyn M, Xu X Am. J. Pathol., 2006;168(6):1879-88. Species: Human Sample Type: Whole Cells Application: ICC Monitoring differentiation of human embryonic stem cells using real-time PCR. Authors: Noaksson K, Zoric N, Zeng X, Rao MS, Hyllner J, Semb H, Kubista M, Sartipy P Stem Cells, 2005;23(10):1460-7. Species: Human Sample Type: Whole Cells Application: ICC Clonal amniotic fluid-derived stem cells express characteristics of both mesenchymal and neural stem cells. Authors: Tsai MS, Hwang SM, Tsai YL, Cheng FC, Lee JL, Chang YJ Biol. Reprod., 2005;74(3):545-51. Species: Human Sample Type: Whole Cells Application: ICC Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages. Authors: Hyslop L, Stojkovic M, Armstrong L, Walter T, Stojkovic P, Przyborski S, Herbert M, Murdoch A, Strachan T, Lako M Stem Cells, 2005;23(8):1035-43. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Nanog Trp153-Val305 Accession # ABZ92376
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1-3).
运输条件	Blue Ice
存放说明	4℃
参考文献	Mitsui, K. et al. (2003) Cell 11:631. Chambers, I. et al. (2003) Cell 113:643. Hart, A.H. et al. (2004) Dev. Dyn. 230:187.

参考图片

Detection of Human Nanog by Western Blot.
Western blot shows lysates of BG01V human embryonic stem cells and Tera‑2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Nanog at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.

Detection of Nanog-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Nanog/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The nanog promoter was detected by standard PCR.

Nanog in BG01V Human Stem Cells. Nanog was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Nanog in BG01V Human Stem Cells-derived Embryoid Body. Nanog was detected in immersion fixed BG01V human embryonic stem cells-derived embryoid body using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Nanog in ADLF1 and FAB2 Stem Cell Lines. Nanog was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.