| 货号 | AF2894-SP |
| 别名 | CANDF7; DKFZp686B04100; ISGF-3; ISGF-3,91kD; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription-1; STAT91 | 全称 | Signal Transducer and Activator of Transcription 1 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.5 µg/mL) Simple Western(5 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human STAT1 when phosphorylated at Y701 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6772 (Human); 20846 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Molecular determinants of plaque size as an indicator of dengue virus attenuation | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide containing human STAT1 Y701 site |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | STAT1 (signal transducer and activator of transcription #1) is an 88 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT1 is associated with type I and II interferon signaling. All STATs contain an N-terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT1 is phosphorylated at Y701 by receptor-associated Janus kinases (JAKs) leading to STAT1 dimerization and subsequent translocation to the nucleus to activate gene transcription. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Phospho-STAT1 in IFN-alpha -treated Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitts lymphoma cell line was unstimulated (light orange filled histogram) or treated with 500 units/mL Recombinant Human IFN‑ alpha (Catalog # 11100-1) for 20 minutes (dark orange filled histogram), then stained with Rabbit Anti-Human/Mouse Phospho‑STAT1 (Y701) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2894) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. | |
Detection of Human Phospho-STAT1 (Y701) by Western Blot. Western blot shows lysates of Daudi human Burkitts lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN-alpha 2a for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT1 (Y701) at approximately 94 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. | |
Detection of Human Phospho-STAT1 (Y701) by Simple WesternTM. Simple Western lane view shows lysates of Daudi human Burkitts lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN‑ alpha A (Catalog # 11100-1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-STAT1 (Y701) at approximately 86 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
