Phospho-JNK (T183/Y185) Affinity Purified PAb (25 UG)【科瑞奥博】

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Phospho-JNK (T183/Y185) Affinity Purified PAb (25 UG)

货号： AF1205-SP 基本售价： 2111.7 元规格： -

产品信息

概述
货号	AF1205-SP
别名	C-jun-amino-terminal kinase interacting protein 3; C-Jun-amino-terminal kinase-interacting protein 3; DKFZp762N1113; homolog of Drosophila Sunday driver 2; JIP-3; JIP3FLJ00027; JNK MAP kinase scaffold protein 3; JNK/SAPK-associated protein-1; JNK/stress-activated protein kinase-associated protein 1; JNK-interacting protein 3; JSAP1; KIAA1066SYD2; mitogen-activated protein kinase 8 interacting protein 3; Mitogen-activated protein kinase 8-interacting protein 3; syd
全称	C-Jun N-terminal Kinase
应用	Western Blot(0.5 µg/mL) Simple Western(5 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human, mouse and rat p46 and p54 JNK when dually phosphorylated at sites homologous to T183/Y185 of JNK1 and JNK2, and T221/Y223 of JNK3 in Western blots.
使用方法	Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. White-to-brown metabolic conversion of human adipocytes by JAK inhibition. Authors: Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C Nat Cell Biol, 2015;17(1):57-67. Species: Human Sample Type: Cell Lysate Application: WB High glucose alters retinal astrocytes phenotype through increased production of inflammatory cytokines and oxidative stress. Authors: Shin E, Huang Q, Gurel Z, Sorenson C, Sheibani N PLoS ONE, 2014;9(7):e103148. Species: Mouse Sample Type: Cell Lysates Application: WB ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes. Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J J Biol Chem, 2012;287(32):26478-94. Species: Human Sample Type: Cell Lysates Application: WB Protective effect of the poly(ADP-ribose) polymerase inhibitor PJ34 on mitochondrial depolarization-mediated cell death in hepatocellular carcinoma cells involves attenuation of c-Jun N-terminal kinase-2 and protein kinase B/Akt activation. Mol. Cancer, 2012;11(0):34. Species: Human Sample Type: Cell Lysates Application: WB Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema. Authors: Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(2):L388-400. Species: Human Sample Type: Cell Lysates Application: WB Common dysregulation of Wnt/Frizzled receptor elements in human hepatocellular carcinoma. Authors: Bengochea A, de Souza MM, Lefrancois L, Le Roux E, Galy O, Chemin I, Kim M, Wands JR, Trepo C, Hainaut P, Scoazec JY, Vitvitski L, Merle P Br. J. Cancer, 2008;99(1):143-50. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen and protein A Affinity-purified
免疫原	Phosphopeptide containing human, rat, and mouse JNK1 T183/Y185 site
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The c-Jun N-terminal Kinases (JNKs) are part of the MAPK (mitogen-activated protein kinase) system that transmits signals from the extracellular milieu to both the cytoplasm and nucleus of the cell. Following perturbation at the cell membrane, MEKKs/MAP3Ks are initially activated, followed by their activation of MKKs/MAP2Ks, and MKKs activation of MAPKs/MAP(1)Ks. There are three classes of MAPKs: ERKs, p38 Kinases and JNKs. JNKs are 45-55 kDa protein products of three genes which, through alternative splicing, generate up to 10 possible isoforms. The phosphorylation targets for MAPKs vary, but include p53, c-MYC, ATF2 and c-Jun, the latter molecule representing the namesake for the enzyme group. The three human JNKs share approximately 80% aa sequence identity. JNKs from human, mouse and rat all contain a conserved Met-Met-Thr(183)-Pro-Tyr(185)-Val-Val motif that undergoes dual phosphorylation by MMK4 and MMK7 to activate the different JNKs. Activated by environmental stresses and inflammatory cytokines, JNKs translocate to the nucleus where they regulate the activity of several transcription factors; including the c-Jun component of AP-1 and ATF-2.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling Apoptosis Intracellular Kinases Cancer Biomarkers Chemokine Receptor Intracellular Signaling C-type Lectin Receptors Diabetic Peripheral Neuropathy EMT Induction GDNF Family ICC / IHC Images: JNK IL-1 Family Interactive Pathway: IL-1 Family Signaling Pathways Intracellular Kinases Intracellular Kinases in the Akt Pathway Intracellular Signaling Molecules in Angiogenesis Neurotrophin/Trk Family NOD-like Receptors and the Inflammasome Positive Regulators of the Jak/STAT Pathway TNF Superfamily Regulators Toll-Like Receptors Wnt Intracellular Signaling

参考图片

JNK in Human Breast Cancer Tissue. JNK phosphorylated at T183/Y185 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1205) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

Detection of Human and Mouse Phospho-JNK (T183/Y185) by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line and NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 J/m² UV-C for 30 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1205), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-JNK (T183/Y185) at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.

JNK in Human Prostate. JNK phosphorylated at T183/Y185 was detected in immersion fixed paraffin-embedded sections of human prostate array using Rabbit Anti-Human/Mouse/Rat Phospho‑JNK (T183/Y185) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1205) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Phospho-JNK (T183/Y185) by Simple Western^TM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 100 J/m² ultraviolet light (UV) for 30 minutes, loaded at 0.2 mg/mL. Specific bands were detected for Phospho-JNK (T183/Y185) at approximately 56 and 46 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1205). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

*Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.