| 货号 | AF2055-SP |
| 别名 | PKB; RAC | 全称 | v-Akt Murine Thymoma Viral Oncogene Homolog |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human Akt1, Akt2, and Akt3 in Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Akt synthetic peptide IRCLQWTTVIERTFHV Accession # P31749 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Rabbit antibodies were raised against a synthetic peptide corresponding to amino acids 75‑90 of human v-akt murine thymoma viral oncogene homolog 1 (Akt1), also known as protein kinase B alpha (PKB alpha ) and RAC-alpha serine/threonine kinase (RAC-PK-alpha ). In addition, this peptide sequence is identical to amino acids 75‑90 of human Akt2 (PKB beta, RAC-PK-beta ), and amino acids 74‑89 of human Akt3 (PKB gamma, RAC-PK-gamma ). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Akt by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, MCF‑7 human breast cancer cell line, and KG‑1 human myeloid leukemia cell lines. PVDF membrane was probed with 0.1 µg/mL of Human Akt Pan Specific, Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2055) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Akt Pan Specific, at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
