Phospho-Tie-2 (Y992) Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Phospho-Tie-2 (Y992) Affinity Purified Polyclonal Ab (25 UG)

货号： AF2720-SP 基本售价： 2072.1 元规格： -

产品信息

概述
货号	AF2720-SP
别名	angiopoietin-1 receptor; CD202b antigen; CD202b; EC 2.7.10; EC 2.7.10.1; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; TEK tyrosine kinase, endothelial; TEK; TIE-2; TIE2CD202b; Tunica interna endothelial cell kinase; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; venous malformations, multiple cutaneous and mucosal; VMCM; VMCM1
全称	Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
应用	Western Blot(0.5 µg/mL) Intracellular Staining by Flow Cytometry(0.25 µg/10⁶cells)
目标/特异性	Detects human and mouse Tie-2 when phosphorylated at Y992 in Western blots.
使用方法	Western Blot: 0.5 µg/mL Intracellular Staining by Flow Cytometry: 0.25 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7010 (Human); 21687 (Mouse); 30747 (Zebrafish)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Tie1 attenuation reduces murine atherosclerosis in a dose-dependent and shear stress-specific manner. Authors: Woo KV, Qu X, Babaev VR, Linton MF, Guzman RJ, Fazio S, Baldwin HS J. Clin. Invest., 2011;121(4):1624-35. Species: Mouse Sample Type: Cell Lysates Application: WB Ectopic expression of angiopoietin-1 promotes neuronal differentiation in neural progenitor cells through the Akt pathway. Authors: Bai Y, Cui M, Meng Z, Shen L, He Q, Zhang X, Chen F, Xiao J Biochem. Biophys. Res. Commun., 2009;378(2):296-301. Species: Mouse Sample Type: Cell Lysates Application: WB Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts. Authors: Saharinen P, Eklund L, Miettinen J, Wirkkala R, Anisimov A, Winderlich M, Nottebaum A, Vestweber D, Deutsch U, Koh GY, Olsen BR, Alitalo K Nat. Cell Biol., 2008;10(5):527-37. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Phosphopeptide containing human Tie-2 Y992 site
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Tie-2 (also known as TEK) is an angiogenic receptor tyrosine kinase required for the later stage of blood vessel maturation. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Y992 is located on the putative activation loop of Tie-2 and is a major autophosphorylation site.
运输条件	Blue Ice
存放说明	4℃
参考文献	Angiopoietins, Angiopoietin-like Proteins, and Receptors Endothelial Cell Differentiation Markers Endothelial Cell Markers Endothelial Progenitor and Endothelial Cell Markers Endothelial Progenitor Cell Markers Hemangioblasts Human CD Marker Products ICC / IHC Images: Tie-2 Receptor Tyrosine Kinases (RTKs) Receptor Tyrosine Kinases (RTKs) in the Akt Pathway Receptors in the Jak/STAT Pathway The Search for Tie-1 and Tie-2 Ligands

参考图片

Detection of Tie‑2 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were unstimulated (light orange filled histogram) or treated with 100 μM pervanadate for 15 minutes (dark orange filled histogram), then stained with Human/Mouse Phospho-
Tie‑2 (Y992) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2720) or control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Detection of Mouse Phospho-Tie‑2 (Y992) by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line transfected with mouse Tie-2 and untreated (-) or treated (+) with
600 ng/mL Recombinant Human Angiopoietin‑1 (Catalog # 923-AN) for 5 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-Tie‑2 (Y992) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2720) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Tie‑2 (Y992) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.