Human Phospho-p53 (S392) Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Phospho-p53 (S392) Affinity Purified Polyclonal Ab (25 UG)

货号： AF2996-SP 基本售价： 2072.1 元规格： -

产品信息

概述
货号	AF2996-SP
别名	Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53
反应种属	Human
应用	Western Blot(0.1 µg/mL) Simple Western(1 µg/mL)
目标/特异性	Reconstitute at 0.2 mg/mL in sterile PBS.
使用方法	Western Blot: 0.1 µg/mL Simple Western: 1 µg/mL
来源	Polyclonal Rabbit IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7157 (Human); 22059 (Mouse); 24842 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Phosphopeptide containing human p53 S392 site
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The p53 tumor suppressor protein acts to enforce cell cycle checkpoints or signal apoptosis in cells that have incurred genotoxic damage. p53 is phosphorylated at serine 392 in response to UV light and other genotoxic stressors. Phosphorylation of p53 at S392 stabilizes p53 tetramers and increases DNA binding activity.
运输条件	Blue Ice
存放说明	4℃
参考文献	Apoptosis Transcription Factors and Regulators Cancer Biomarkers Cancer Stem Cell Transcription Factors Cell Cycle Checkpoint DNA Damage and Repair Epithelial Stem Cell Transcription Factors Hematopoietic Stem Cell Transcription Factors and Regulators ICC / IHC Images: p53 Immunoglobulin-Like Domain Transcription Factors Mesenchymal Stem Cell Transcription Factors Mini-review: DNA Damage Response Mini-review: Genomic Instability Syndromes Neural Stem Cell Transcription Factors p53 Pathway p53: More than a Transcription Factor? Parkinsons Disease Related Molecules Pluripotent Stem Cell Transcription Factors TNF Superfamily Regulators Transcription Factors and Regulators Transcription Factors in the Akt Pathway

参考图片

Detection of Human Phospho-p53 (S392) by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 5 hours. PVDF membrane was probed with 0.1 µg/mL Rabbit Anti-Human Phospho-p53 (S392) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2996) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S392) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for 1 hour. For additional reference, the membrane was stripped and reprobed with 1:5000 dilution of Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Phospho-p53 (S392) by Simple Western^TM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 5 hours, loaded at 0.5 mg/mL. A specific band was detected for Phospho-p53 (S392) at approximately 62 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human Phospho-p53 (S392) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2996). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.