Human Phospho-p53 (S37) Affinity Purified Polyclonal Ab (25 UG)
货号：
AF3306-SP 基本售价：
2072.1 元 规格：
-
产品信息
概述| 货号 | AF3306-SP |
| 别名 | Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53 |
| 反应种属 | Human |
| 应用 | Western Blot(0.4 µg/mL)
|
| 目标/特异性 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 使用方法 | Western Blot: 0.4 µg/mL
|
| 来源 | Polyclonal Rabbit IgG |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 7157 (Human); 22059 (Mouse); 24842 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide containing human p53 S37 site |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The p53 tumor suppressor protein acts to enforce cell cycle checkpoints or signal apoptosis in cells that have incurred genotoxic damage. The ATR and DNA-PKCS kinases phosphorylate p53 at serine 37 in response to UV light and other genotoxic stressors. This phosphorylation event results in increased p53 transcriptional activation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Apoptosis Transcription Factors and Regulators
- Cancer Biomarkers
- Cancer Stem Cell Transcription Factors
- Cell Cycle
- Checkpoint
- DNA Damage and Repair
- Epithelial Stem Cell Transcription Factors
- Hematopoietic Stem Cell Transcription Factors and Regulators
- ICC / IHC Images: p53
- Immunoglobulin-Like Domain Transcription Factors
- Mesenchymal Stem Cell Transcription Factors
- Mini-review: DNA Damage Response
- Mini-review: Genomic Instability Syndromes
- Neural Stem Cell Transcription Factors
- p53 Pathway
- p53: More than a Transcription Factor?
- Parkinsons Disease Related Molecules
- Pluripotent Stem Cell Transcription Factors
- TNF Superfamily Regulators
- Transcription Factors and Regulators
- Transcription Factors in the Akt Pathway
|
参考图片Detection of Human Phospho-p53 (S37) by Western Blot. Western blot shows p53 immunoprecipitated from lysates of MCF‑7 human breast cancer cell line using Human/Mouse/Rat p53 Agarose-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # GAF1355). MCF‑7 cell line was untreated (-) or treated (+) with 1 μM camptothecin (CPT) for 5 hours. PVDF membrane was probed with 0.4 µg/mL Rabbit Anti-Human Phospho-p53 (S37) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3306) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S37) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for 1 hour. For additional reference the membrane was stripped and reprobed with 1:5000 dilution of Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody ( lower panel, Catalog # HAF1355) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
