概述| 货号 | AF850-SP |
| 别名 | APP+1 |
全称 | Amyloid Precursor Protein +1 Frameshift Mutant | | 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL)
|
| 目标/特异性 | Detects human APP+1 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL
|
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 351 (Human); 11820 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | KLH-coupled human APP+1 synthetic peptide
CMRMGRGRTSSKELA |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Amyloid Precursor Protein (APP) is a type I transmembrane protein that is ubiquitously expressed on cell surfaces. It undergoes complex proteolytic processing and is cleaved by alpha-, beta-, and gamma-Secretases to generate soluble APP alpha, soluble APP beta, and Amyloid beta (A beta) fragments of several lengths. One of these fragments, A beta 42, generated by beta- and gamma-Secretase activities, has been implicated in Alzheimers disease. Aberrantly high levels of this peptide form and accumulate in the brains of Alzheimers disease patients to create the senile plaques characteristic of the disease. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Acute Phase Proteins
- APP Cleavage and Amyloid-beta Degradation
- Neurodegenerative Disease
|
参考图片Detection of Human APP+1 by Western Blot. Western blot shows samples of Recombinant Human APP +1 Western Blot Standard (Catalog # WBC012) (??? ng). ??? membrane was probed with 0.5 µg/mL Rabbit Anti-Human APP +1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF850) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for APP was detected at approximately ??? kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4. |
