Phospho-GSK-3 alpha/beta (S21/S9) Affinity Purified PAb (25 UG)【科瑞奥博】

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Phospho-GSK-3 alpha/beta (S21/S9) Affinity Purified PAb (25 UG)

货号： AF1590-SP 基本售价： 1407.8 元规格： -

产品信息

概述
货号	AF1590-SP
别名	DKFZp686D0638; EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 alpha; glycogen synthase kinase-3 alpha; GSK-3 alpha
全称	Glycogen Synthase Kinase 3
应用	Western Blot(0.2 µg/mL) Immunocytochemistry(5-15 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human, mouse, and rat GSK-3 beta when phosphorylated at S9, and human, mouse, and rat GSK‑3 alpha when phosphorylated at S21.
使用方法	Western Blot: 0.2 µg/mL Immunocytochemistry: 5-15 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. HYS-32-Induced Microtubule Catastrophes in Rat Astrocytes Involves the PI3K-GSK3beta Signaling Pathway. Authors: Chiu C, Liao C, Shen C, Tang T, Jow G, Wang H, Wu J PLoS ONE, 2015;10(5):e0126217. Species: Rat Sample Type: Cell Lysates Application: WB Mechanical induction of PGE2 in osteocytes blocks glucocorticoid-induced apoptosis through both the beta-catenin and PKA pathways. Authors: Kitase Y, Barragan L, Qing H, Kondoh S, Jiang JX, Johnson ML, Bonewald LF J. Bone Miner. Res., 2011;25(12):2381-92. Species: Mouse Sample Type: Cell Lysates Application: WB Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation. Authors: Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, Wang B, Wardega P, Zhang F, Dong L, Zhang Y, Zhang SZ, Ding H, Fariss RN, Becker KG, Lennartsson J, Nagai N, Cao Y, Li X J. Exp. Med., 2010;207(4):867-80. Species: Rat Sample Type: Cell Lysates Application: WB
纯化方式	Antigen and protein A Affinity-purified
免疫原	Phosphopeptide containing GSK-3 beta S9 site
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	GSK-3 is a Ser/Thr kinase first identified as an inactivator of Glycogen Synthase. GSK-3 acts as a multifunctional downstream switch that determines the output of numerous signaling pathways. There are two mammalian GSK-3 isoforms encoded by distinct genes, GSK-3 alpha and GSK-3 beta, which are structurally similar, but functionally non-identical. GSK-3a is inhibited by phosphorylation at S21 by Akt and other kinases. GSK-3 alpha and GSK-3 beta share 85% amino acid identity. Dysregulated GSK-3 has been implicated in several diseases including type II diabetes, Alzheimers disease, bipolar disorder, and cancer.
运输条件	Blue Ice
存放说明	4℃
参考文献	Apoptosis Intracellular Kinases Common gamma Chain Receptor Family Hedgehog Family ICC / IHC Images: GSK-3 alpha/beta Intracellular Kinases in the Akt Pathway Intracellular Signaling Molecules in Angiogenesis Notch Pathway Wnt Intracellular Signaling

参考图片

Detection of Phospho-GSK‑3 alpha / beta (S21/S9) in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cells, untreated (open histogram, yellow line), or treated with PI3-kinase inhibitors (dark orange filled histogram), were stained with Human/Mouse/Rat Phospho-GSK‑3 alpha / beta (S21/S9) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF1590) or control antibody (Catalog # AB-105-C, blue open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Detection of Human Phospho-GSK‑3 alpha (S21) and GSK-3 beta (S9) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog #291‑G1) for 15 minutes. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat Phospho-GSK‑3 alpha / beta (S21/S9) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1590), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-GSK‑3 alpha (S21) and GSK-3 beta (S9) at approximately 51 and 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.