Phospho-GSK-3 alpha (S21) Affinity Purified PAb (25 UG)【科瑞奥博】

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Phospho-GSK-3 alpha (S21) Affinity Purified PAb (25 UG)

货号： AF4125-SP 基本售价： 1407.8 元规格： -

产品信息

概述
货号	AF4125-SP
别名	DKFZp686D0638; EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 alpha; glycogen synthase kinase-3 alpha; GSK-3 alpha; GSK3A
全称	Glycogen Synthase Kinase 3 alpha
应用	Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human, mouse, and rat GSK-3 alpha when phosphorylated at S21. This antibody does not detect phosphorylated GSK-3 beta.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2931 (Human); 606496 (Mouse); 50686 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Mechanism and management of AKT inhibitor-induced hyperglycemia. Authors: Crouthamel MC, Kahana JA, Korenchuk S, Zhang SY, Sundaresan G, Eberwein DJ, Brown KK, Kumar R Clin. Cancer Res., 2009;15(1):217-25. Species: Mouse Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Phosphopeptide containing human, mouse, and rat GSK-3 alpha S21 site
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 alpha is inhibited by phosphorylation at S21 by Akt and other kinases. Deregulated GSK-3 has been implicated in several diseases including type II diabetes, Alzheimer’s disease, bipolar disorder, and cancer.
运输条件	Blue Ice
存放说明	4℃
参考文献	Apoptosis Intracellular Kinases Common gamma Chain Receptor Family Hedgehog Family ICC / IHC Images: GSK-3 alpha Intracellular Kinases Intracellular Kinases in the Akt Pathway Intracellular Signaling Molecules in Angiogenesis Notch Pathway Wnt Family Wnt Intracellular Signaling

参考图片

GSK‑3 alpha in Human Thymus. GSK‑3 alpha was detected in immersion fixed paraffin-embedded sections of human thymus using 5 µg/mL Human/Mouse/Rat Phospho-GSK‑3 alpha (S21) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4125) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human and Mouse Phospho-GSK‑3 alpha (S21) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog #291-G1) for 15 minutes and NIH‑3T3 mouse embryonic fibroblast cell line untreated or treated with 10 ng/mL PMA for 20 minutes. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Phospho-GSK‑3 alpha (S21) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4125), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-GSK‑3 alpha (S21) at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.