Phospho-MSK2/RSKB (S196) Affinity Purified PAb (25 UG)【科瑞奥博】

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Phospho-MSK2/RSKB (S196) Affinity Purified PAb (25 UG)

货号： AF189-SP 基本售价： 1407.8 元规格： -

产品信息

概述
货号	AF189-SP
别名	ribosomal protein S6 kinase, 90kDa, polypeptide 4; RPS6KA4; RSKB
全称	Mitogen- and Stress-activated Protein Kinase 2
应用	Western Blot(0.5 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human and mouse MSK2/RSKB when phosphorylated at S196.
使用方法	Western Blot: 0.5 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8986 (Human); 56613 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Mitogen- and stress-activated protein kinase 2 and cyclic AMP response element binding protein are activated in lesional psoriatic epidermis. Authors: Funding AT, Johansen C, Kragballe K, Iversen L J. Invest. Dermatol., 2007;127(8):2012-9. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Dimethylfumarate specifically inhibits the mitogen and stress-activated kinases 1 and 2 (MSK1/2): possible role for its anti-psoriatic effect. Authors: Gesser B, Johansen C, Rasmussen MK, Funding AT, Otkjaer K, Kjellerup RB, Kragballe K, Iversen L J. Invest. Dermatol., 2007;127(9):2129-37. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Phosphopeptide containing human and mouse MSK2/RSKB S196 site
生物活性	Human, Mouse
标记	Unconjugated
背景	Mitogen- and stress-activated protein kinase 1 (MSK1), also known as ribosomal protein S6 kinase 5 (RPS6KA5), belongs to the AGC family of kinases. Both MSK1 and the related MSK2, also known as RSKB and RPS6KA4, have two kinase domains connected by a regulatory linker region, and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.
运输条件	Blue Ice
存放说明	4℃
参考文献	Apoptosis Intracellular Kinases ICC / IHC Images: MSK2 Intracellular Kinases Stem Cell Epigenetics

参考图片

MSK2/RSKB in Human Pancreas. MSK2/RSKB was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human/Mouse Phospho-MSK2/RSKB (S196) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF189) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Phospho-MSK2/RSKB (S196) by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human HGF (Catalog # 294-HG) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-MSK2/RSKB (S196) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF189), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.