Human Caspase-10 (FLICE 2) Affinity Purified PAb (25 UG)【科瑞奥博】

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Human Caspase-10 (FLICE 2) Affinity Purified PAb (25 UG)

货号： AF834-SP 基本售价： 1407.8 元规格： -

产品信息

概述
货号	AF834-SP
别名	Apoptotic protease Mch-4; CASP10; CASP-10; caspase 10, apoptosis-related cysteine peptidase; caspase 10, apoptosis-related cysteine protease; caspase-10; EC 3.4.22.63; FADD-like ICE2; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE2; ICE-like apoptotic protease 4; interleukin-1B-converting enzyme 2; Mch4; MCH4ALPS2
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human and mouse Caspase-10 (FLICE 2) precursor in Western blots. Sensitivity for detection of the small subunit is not known.
使用方法	Western Blot: 1 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	843 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The B30.2 domain of pyrin, the familial Mediterranean fever protein, interacts directly with caspase-1 to modulate IL-1beta production. Authors: Chae JJ, Wood G, Masters SL, Richard K, Park G, Smith BJ, Kastner DL Proc. Natl. Acad. Sci. U.S.A., 2006;103(26):9982-7. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	KLH-coupled human Caspase-10 (FLICE 2) synthetic peptide KRTVWGAKQISATSLPTAC
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Caspase-10 (Cysteine-aspartic acid protease 10, FLICE 2 and Mch4) is a member of the peptidase C14A family of enzymes. It is widely expressed, being found in blood and epithelial cells. Caspase-10 appears to be an initiator caspase that acts on Pro-Caspases-3, -4, -6, -7, and -9. Human Pro-Caspase-10a is a 59 kDa, 521 amino acid (aa) protein. It contains two N-terminal death domains. Cleavage generates p43 plus the small mature p12 subunit. Cleavage of p43 generates a prodomain p25 and large mature p17 subunit. P17 and p12 noncovalently associate to form a 29 kDa mature heterodimer. There are at least four isoform variants. There is no apparent mouse Casp-10 gene.
运输条件	Blue Ice
存放说明	4℃
参考文献	Apoptosis: Caspase Pathways Apoptosis: Mitochondria Accused of Murder Caspases and Regulators Cysteine Proteases and Regulators ICC / IHC Images: Caspase-10 Interactive Pathway: Apoptosis Signaling Pathways Mini-review: Caspases Novel Caspase-8 Roles Revealed by Knockouts RIG-I-like Receptors Technical Notes: Caspase Antibodies TNF Superfamily Regulators

参考图片

Detection of Human Caspase‑10 (FLICE 2), by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Caspase‑10 (FLICE 2) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF834) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Caspase‑10 (FLICE 2), at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.