| 货号 | AF7677-SP |
| 别名 | cadherin 15, M-cadherin (myotubule); cadherin 15, type 1, M-cadherin (myotubule); Cadherin-14; Cadherin-15; cadherin-3; CDH14; CDH14cadherin-14; CDH15; CDH3cadherin-15; CDHM; MCAD; M-cadherin; MRD3; Muscle cadherin; muscle-cadherin | 全称 | M-Cadherin [Myotubule] |
| 反应种属 | Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse and rat M‑Cadherin/Cadherin‑15 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human M‑Cadherin/Cadherin‑15 is observed, and less than 1% cross-reactivity with recombinant mouse P-Cadherin is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1013 (Human); 12555 (Mouse); 361432 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Impairment of cold injury-induced muscle regeneration in mice receiving a combination of bone fracture and alendronate treatment | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse M‑Cadherin/Cadherin‑15 Val22-Ala605 (Arg159His) Accession # P33146 |
| 生物活性 | Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | CDH-15 (Cadherin 15; also M-cadherin, Muscle cadherin and Cadherin-14) is a 125-127 kDa atypical member of the classical cadherin family, cadherin superfamily of molecules. It is expressed by muscle satellite cells, cells of the embryonic myotome, and hematopoietic bone marrow stem cells. CDH-15 appears to bind homotypically in trans, thus allowing for the identification and subsequent fusion of myoblast precursors, particularly those in slow-twitch (or red fiber) muscles. This is accompanied by a downregulation of mitochondrial induced apoptosis. Mouse CDH-15 is synthesized as a 784 amino acid (aa) preproprecursor. It contains a 21 aa signal sequence, a 38 aa propeptide, and a 725 aa mature region. The mature region is expressed as a type I transmembrane glycoprotein that possesses a 546 aa extracellular region (aa 60-605) and a 159 aa cytoplasmic domain (aa 626-784). The extracellular region shows five consecutive cadherin domains. Over aa 22-605, mouse CDH-15 shares 88% and 97% aa sequence identity with human and rat CDH-15, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse and Rat M‑Cadherin/ Cadherin‑15 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, mouse brain (cerebellum) tissue, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat M‑Cadherin/Cadherin‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7677) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for M‑Cadherin/Cadherin‑15 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
