| 货号 | AF733-SP |
| 别名 | desert hedgehog (Drosophila) homolog; desert hedgehog homolog; desert hedgehog protein; desert hedgehog; DHH; HHG-3MGC35145 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Desert Hedgehog/Dhh N-Terminus in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse (rm) Shh N-terminal peptide (aa 25‑198) is observed. Additionally, in direct ELISAs, no cross‑reactivity with rmDhh C-terminal peptide (aa 199‑396) and rmIhh C-terminal peptide (aa 241‑449) is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 50846 (Human); 13363 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Desert Hedgehog/Dhh aa 23-198 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Dhh regulates testis development and spermatogenesis via interaction of its N-terminal domain with the receptors Patched and Patched-2. Hedgehog proteins are secreted as precursor molecules that undergo autocatalytic internal cleavage to yield an N-terminal domain that has signaling activity and a C-terminal domain that is active in precursor processing. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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