| 货号 | AF6184-SP |
| 别名 | AIRE; APECED protein; APECED; APECEDAPSI; APS1; APSI; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; autoimmune regulator (APECED protein)10APS1AIRE1; autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermaldystrophy); autoimmune regulator; PGA1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse Autoimmune Regulator/AIRE in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 326 (Human); 11634 (Mouse); 294328 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Autoimmune Regulator/AIRE Ser476-Ser552 Accession # Q9Z0E3 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | AIRE (Autoimmune regulator; also APECED) is a 58-60 kDa transcriptional regulator that exhibits tight regulation of its expression. It is widely expressed in lymphoid organs, but is most pronounced in thymic medullary epithelium, where it induces the expression of organ specific antigens that are used to sequester and delete autoreactive lymphocytes. Mouse AIRE is 552 amino acids (aa) in length. It contains multiple domains, including an HSR domain with two LXXLL motifs (aa 1-106), an NLS (aa 114-134), one SAND domain (aa 182-282) and two PHD zinc-finger regions (aa 298-345; 434-475). AIRE will homodimerize and tetramerize. There are multiple potential isoforms that involve various combinations of deletions of aa 265-268, 368-426 and Lys296, plus a 42 aa substitution for aa 368-552. Over aa 476-552, mouse AIRE shares 91% and 65% aa identity with rat and human AIRE, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse Autoimmune Regulator/AIRE by Western Blot. Western blot shows lysates of mouse thymus tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse Autoimmune Regulator/AIRE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6184) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Autoimmune Regulator/AIRE at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
