Mouse alpha 1-Acid Glycoprotein Affinity Purified PAb (25 UG)
货号：
AF5934-SP 基本售价：
1407.8 元 规格：
-
产品信息
概述| 货号 | AF5934-SP |
| 别名 | AGP 1; AGP; AGP1; AGP1alpha-1-acid glycoprotein 1; AGPA; AGP-A; OMD 1; ORM; ORM1; orosomucoid 1; Orosomucoid; orosomucoid-1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL)
|
| 目标/特异性 | Detects mouse alpha 1‑Acid Glycoprotein in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with human alpha 1‑Acid Glycoprotein is observed. |
| 使用方法 | Western Blot: 1 µg/mL
|
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 5004 (Human); 18405 (Mouse); 24614 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse plasma-derived alpha 1‑Acid Glycoprotein
Accession # P02763 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | alpha 1-Acid Glycoprotein (AGP; also OMD/Orosomucoid) is a 40‑46 kDa member of the immunocalin subfamily, lipocalin family of molecules. In mouse, circulating AGP is principally the product of hepatocytes that originates from multiple related genes (AGP-1, -2 & -3 in Mus musculus). Circulating AGP-1 and -2 are both 189 amino acids (aa) in length, the principal sources of protein, and show 83% aa identity; AGP-3 contributes little to the AGP pool. In mouse blood, AGP is normally 200‑400 μg/mL. In response to inflammatory mediators (IL-6; IL-1), its concentration will rise 2 to 10 fold. More importantly, a complex glycosylation pattern will also change, transitioning from modestly branched to highly branched oligosaccharides. This change is reflected in its bioactivity, which has been shown to be a function of carbohydrate branching. AGP is generally considered to be a suppressor of inflammation. Rat and human AGP share only 70% and 47% aa identity with mouse AGP, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Acute Phase Proteins
- ICC / IHC Images: alpha 1-Acid Glycoprotein
- Inflammatory Mediators
|
参考图片Detection of Mouse alpha 1-Acid Glycoprotein by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse alpha 1‑Acid Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5934) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha 1‑Acid Glycoprotein at approximately 43kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
