| 货号 | AF5236-SP |
| 别名 | BTL-II; butyrophilin-like 2 (MHC class II associated); Butyrophilin-like 2 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse BTNL2/Butyrophilin-like 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant mouse (rm) BTN, rmBTN2, and recombinant human BTN is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 56244 (Human); 547431 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse BTNL2/Butyrophilin-like 2 aa 27-452 Accession # O70355 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Butyrophilin-like 2 (BTNL2; also BTL-II) is a predicted 60‑65 kDa member of the BTN/MOG family, Ig-superfamily of proteins. In mouse, it is expressed in intestinal villi columnar epithelial cells and colonic dendritic cells. BTNL2 apparently blocks CD4+ T cell activation. Mouse BTNL2 is a 454 amino acid (aa) type II transmembrane glycoprotein. It contains a 431 aa extracellular region (aa 24‑454) with four V-type Ig-like domains. There are three splice variants. One shows a deletion of the two C-terminal amino acids, a second shows a deletion of aa 237‑243, while a third shows a C-terminal 62 substitution for Gly453Trp454. Over aa 27‑452, mouse BTNL2 shares 88% and 64% aa identity with rat and human BTNL2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse BTNL2/Butyrophilin-like 2 by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse BTNL2/Butyrophilin-like 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5236) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BTNL2/Butyrophilin-like 2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
