| 货号 | AF4636-SP |
| 别名 | AMY; AMYolfactomedin related ER localized protein; neuroblastoma protein; Neuronal olfactomedin-related ER localized protein; NOE1; NOE1noelin; NOEL1; NOELIN; NOELIN1; Noelin-1; OlfA; olfactomedin 1; olfactomedin-1; OLFM1; pancortin 1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects mouse Olfactomedin‑1/Noelin‑1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10439 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Olfactomedin-1 activity identifies a cell invasion checkpoint during epithelial-mesenchymal transition in the chick embryonic heart. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Olfactomedin‑1/Noelin‑1 Ser2-Arg481 Accession # NP_062371 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Olfactomedin‑1 (neuronal olfactomedin-related endoplasmic reticulum-localized-1), also known as Olfactomedin-1 or pancortin, is a 75 kDa secreted extracellular matrix glycoprotein expressed in the brain (1‑3). Alternate promoter usage and splicing creates four forms of Noelin (Noelin 1‑4) that are combinations of a common central region with alternate N- and C-termini (3, 4). Noelin-1 is the longest of the four isoforms, encoding a 16 amino acid (aa) signal sequence and a 469 aa mature protein with a coiled-coil region for multimerization and an olfactomedin-like domain. The shortest isoforms, Noelin-3 and -4, are truncated within the coiled-coil region and lack the olfactomedin-like domain (4). In the species tested, these isoforms appear to oppose actions of the longer isoforms (5). All isoforms are capable of forming homo‑ or heterodimers through cysteines in their common central region (2). The C‑terminus of Noelin-1 (mouse aa 482‑485) contains a putative endoplasmic reticulum retention sequence that has not been included in the R&D Systems protein (6). Mouse Noelin-1 (aa 17‑481) shares > 99%, 99%, 97%, 96% and 93% aa identity with corresponding regions of rat, human, opossum, chicken and Xenopus Noelin-1, respectively. In vivo expression patterns differ somewhat between species (1). In chick early development, Noelin-1 shows highest concentration in areas that produce neural crest cells and is produced by migrating neural crest cells (7). Overproduction of Noelin-1 prolongs neural crest cell formation (7). Noelin-1 is also expressed in mouse neural crest, but not frog (1‑3). All three species express Noelin-1 later in differentiating neural tissues in the spinal cord, brain and cranial ganglia (1‑3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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