| 货号 | AF2985-SP |
| 别名 | EC 3.4.19.6; FLJ22381; PAP-II; PAP-IIthyroliberinase; PGPEP2; Pyroglutamyl-peptidase II; Thyroliberinase; thyrotropin-releasing hormone degrading enzyme; thyrotropin-releasing hormone-degrading ectoenzyme; TRHDE; TRH-DE; TRH-degrading ectoenzyme; TRH-DEthyrotropin-releasing hormone degrading ectoenzyme; TRH-specific aminopeptidase | 全称 | Thyrotropin Releasing Hormone-degrading Ectoenzyme |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse and rat TRH-degrading Ectoenzyme/TRHDE in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 29953 (Human); 237553 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. NMDA receptor up-regulates pyroglutamyl peptidase II activity in the rat hippocampus. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse TRH-degrading Ectoenzyme/TRHDE Arg64-His1025 Accession # Q8K093 |
| 生物活性 | Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TRHDE, also known as pyroglutamyl peptidase II and thyroliberinase, is a metalloprotease that specifically removes pyroglutamate from thyrotropin-releasing hormone, a tripeptide of L-pyroglutamyl-L-histidyl-L-prolineamide. TRH functions as a hypothalamic hypophysiotropic neuropeptide and neurotransmitter/neuromodulator within the central nervous system (1). Inhibitors of TRHDE have potential applications as research and therapeutic agents because TRHDE inactivates TRH (2). TRHDE is a type II transmembrane protein and a soluble form is also present in the serum (1). The recombinant mouse TRHDE corresponds to the ectodomain of the enzyme. Its amino acid sequence is 97% and 95% identical to that of rat and human. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse and Rat TRH-degrading Ectoenzyme/ TRHDE by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line and embryonic rat brain hippocampal glial. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat TRH-degrading Ectoenzyme/TRHDE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2985) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TRH-degrading Ectoenzyme/TRHDE at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
