| 货号 | AF2634-SP |
| 别名 | Prss32 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects mouse Tryptase‑5/Prss32 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Tryptase-G1, recombinant mouse (rm) MCPT-6, and rmMCPT‑7 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Mouse Tryptase‑5/Prss32 (2 µg/mL, Catalog # 2634-SE) cleavage of the fluorogenic peptide substrate Z-R-SBzl (100 µM). The Neutralization Dose (ND50) is typically 24.3 µg/mL. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 69814 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Tryptase‑5/Prss32 Ser20-Arg304 Accession # NP_081496 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Tryptase-5 is encoded by Prss32, one of 13 genes on mouse chromosome 17A3.3 that correspond to functional trypsin-like serine proteases (1). The deduced amino acid sequence of mouse Tryptase-5 consists of 331 residues with a signal peptide (residues 1 to 19), a pro region (residue 20 to 53), a catalytic domain (54 to 304), and a C-terminal hydrophobic peptide (residues 305 to 331). The mRNA is expressed in smooth muscle, eye, stomach, uterus and lymph node. Apparently, Prss32 does not seem to have a counterpart in the human genome. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|